NEET MDS Synopsis
Soft Oral Tissues
Dental Anatomy
Soft Oral Tissues
Oral Mucosa
The oral mucosa consists mainly of two types of tissues: the oral epithelium, which consists of stratified, squamous epithelium, and the underlying connective tissue layer, known as the lamina propria. There are three variations of oral mucosa.
A. Oral epithelium
1. Consists of stratified, squamous epithelium.
2. Four layers (Note: Cells mature as they progress from the deepest [basal] layer to the most superficial [cornified] layer) a. Basal layer (stratum germinativum or basale)
(1) A single layer of cuboidal or columnar cells overlying the lamina propria.
(2) Contains progenitor cells and thus provides cells to the epithelial layers above.
(3) Site of cell division (mitosis).
b. Prickle cell layer (stratum spinosum)
(1) Consists of several layers of larger, ovoid-shaped cells.
c. Granular layer (stratum granulosum)
(1) Cells appear larger and flattened.
(2) Granules (known as keratohyaline granules) are present in the cells.
(3) This layer is absent in nonkeratinized epithelium.
d. Cornified layer (stratum corneum, keratin, or horny layer)
(1) In keratinized epithelium:
(a) Orthokeratinized epithelium the squamous cells on the surface appear flat and contain keratin. They have no nuclei present.
(b) Parakeratinized epithelium the squamous cells appear flat and contain keratin; nuclei are present within the cells.
(2) In parakeratinized epithelium, both squamous cells without nuclei and cells with shriveled (pyknotic) nuclei are present.
(3) In nonkeratinized epithelium, the cells appear slightly flattened and contain nuclei.
B. Lamina propria
1. Consists of type I and III collagen, elastic fibers, and ground substance. It also contains many cell types, including fibroblasts, endothelial cells, immune cells, and a rich vascular and nerve supply.
2. Two layers:
a. Superficial, papillary layer
(1) Located around and between the epithelial ridges.
(2) Collagen fibers are thin and loosely arranged.
b. Reticular layer
(1) Located beneath the papillary layer.
(2) Collagen fibers are organized in thick, parallel bundles.
C. Types of oral mucosa
1. Masticatory mucosa
a. Found in areas that have to withstand compressive and shear forces.
b. Clinically, it has a rubbery, firm texture.
c. Regions: gingiva, hard palate.
2. Lining mucosa
a. Found in areas that are exposed to high levels of friction, but must also be mobile and distensible.
b. Clinically, it has a softer, more elastic texture.
c. Regions: alveolar mucosa, buccal mucosa, lips, floor of the mouth, ventral side of the tongue, and soft palate.
3. Specialized mucosa
a. Similar to masticatory mucosa, specialized mucosa is able to tolerate high compressive
and shear forces; however, it is unique in that it forms lingual papillae.
b. Region: dorsum of the tongue.
D. Submucosa
1. The connective tissue found beneath the mucosa . It contains blood vessels and nerves and may also contain fatty tissue and minor salivary glands.
2. Submucosa is not present in all regions of the oral cavity, such as attached gingiva, the tongue, and hard palate. Its presence tends to increase the mobility of the tissue overlying it.
E. Gingiva
1. The portion of oral mucosa that attaches to the teeth and alveolar bone.
2. There are two types of gingiva: attached and free gingiva. The boundary at which they meet is known as the free gingival groove .
a. Attached gingiva
(1) Directly binds to the alveolar bone and tooth.
(2) It extends from the free gingival groove to the mucogingival junction.
b. Free gingiva
(1) Coronal to the attached gingiva, it is not bound to any hard tissue.
(2) It extends from the gingival margin to the free gingival groove.
c. Together, the free and attached gingiva form the interdental papilla.
.F. Alveolar mucosa
1. The tissue just apical to the attached gingiva.
2. The alveolar mucosa and attached gingiva meet at the mucogingival junction .
G. Junctional epithelium
1. Area where the oral mucosa attaches to the tooth, forming the principal seal between the oral cavity and underlying tissues.
2. Is unique in that it consists of two basal lamina, an internal and external . The internal basal lamina, along with hemidesmosomes, comprises the attachment apparatus (the epithelial attachment). This serves to attach the epithelium directly to the tooth.
3. Histologically, it remains as immature, poorly differentiated tissue. This allows it to maintain its ability to develop hemidesmosomal attachments.
4. Has the highest rate of cell turnover of any oral mucosal tissue.
H. Interdental papilla (interdental gingiva)
1. Occupies the interproximal space between two teeth. It is formed by free and attached gingiva.
2. Functions to prevent food from entering the (interproximal) area beneath the contact point of two adjacent teeth. It therefore plays an important role in maintaining the health of the gingiva.
3. Col
a. If the interdental papilla is cross-sectioned in a buccolingual plane, it would show two peaks (buccal and lingual) with a dip between them, known as the col or interdental col. This depression occurs around the contact point of the two adjacent teeth.
b. Histologically, col epithelium is the same as junctional epithelium
Autoimmune Diseases
General Pathology
Autoimmune Diseases
These are a group of disease where antibodies (or CMI) are produced against self antigens, causing disease process.
Normally one's immune competent cells do not react against one's own tissues.
This is due to self tolerance acquired during embryogenesis. Any antigen encountered at
that stage is recognized as self and the clone of cells capable of forming the corresponding antibody is suppressed.
Mechanism of autoimmunity
(1) Alteration of antigen
-Physicochemical denaturation by UV light, drugs etc. e.g. SLE.
- Native protein may turn antigenic when a foreign hapten combines with it, e.g. Haemolytic anemia with Alpha methyl dopa.
(2) Cross reaction: Antibody produced against foreign antigen may cross react with native protein because of partial similarity e.g. Rheumatic fever.
(3) Exposure of sequestered antigens: Antigens not normally exposed to immune competent cells are not accepted as self as tolerance has not been developed to them. e.g. thyroglobulin, lens protein, sperms.
(4) Breakdown of tolerance :
- Emergence of forbidden clones (due to neoplasia of immune system as in lymphomas and lymphocytic leukaemia)
- Loss of suppressor T cells as in old age and CMI defects
Autoimmunity may be
- Organ specific.
- Non organ specific (multisystemic)
I. Organ specific.
(I) Hemolytic anaemia:
- Warm or cold antibodies (active at 37° C or at colder temperature)
- They may lyse the RBC by complement activation or coat them and make them vulnerable to phagocytosis
(ii) Hashimoto's thyroiditis:
- Antibodies to thyroglobulin and microsomal antigens.
- Cell mediated immunity.
- Leads to chronic. destructive thyroiditis.
(3) Pernicious anemia
Antibodies to gastric parietal cells and to intrinsic factor.
2. Non organ specific.
Lesions are seen in more than one system but principally affect blood vessels and connective tissue (collagen diseases).
(I) Systemic lupus erythematosus (SLE). Antibodies to varied antigens are seen. Hence it is possible that there is abnormal reactivity of the immune system in self recognition.
Antibodies have been demonstrated against:
- Nuclear material (antinuclear I antibodies) including DNA. nucleoprotein etc. Anti nuclear antibodies are demonstrated by LE cell test.
- Cytoplasmic organelles- mitochondria, rib osomes, Iysosomes.
- Blood constituents like RBC, WBC. platelets, coagulation factors.
Mechanism. Immune complexes of body proteins and auto antibodies deposit in various organs and cause damage as in type III hypersensitivity
Organs involved
- Skin- basal dissolution and collagen degeneration with fibrinoid vasculitis.
- Heart- pancarditis.
- Kidneys- glomerulonephritis of focal, diffuse or membranous type
- Joints- arthritis.
- Spleen- perisplenitis and vascular thickening (onion skin).
- Lymph nodes- focal necrosis and follicular hyperplasia.
- Vasculitis in other organs like liver, central or peripheral nervous system etc,
2. Polyarteritis nodosa. Remittant .disseminated necrotising vasculitis of small and medium sized arteries
Mechanism :- Not definitely known. Proposed immune reaction to exogenous or auto antigens
Lesion : Focal panarteritis- a segment of vessel is involved. There is fibrinoid necrosis with initially acute and later chronic inflammatory cells. This may result in haemorrhage and aneurysm.
Organs involved. No organ or tissue is exempt but commonly involved organs are :
- Kidneys.
- Heart.
- Spleen.
- GIT.
3. Rheumatoid arthritis. A disease primarily of females in young adult life.
Antibodies
- Rheumatoid factor (An IgM antibody to self IgG)
- Antinuclear antibodies in 20% patients.
Lesions
- Arthritis which may progress on to a crippling deformity.
- Arteritis in various organs- heart, GIT, muscles.
- Pleuritis and fibrosing alveolitis.
- Amyloidosis is an important complication.
4. Sjogren's Syndrome. This is constituted by
- Kerato conjunctivitis sicca
- Xerostomia
- Rheumatoid arthritis.
Antibodies
- Rheumatoid factor
- Antinuclear factors (70%).
- Other antibodies like antithyroid, complement fixing Ab etc
- Functional defects in lymphocytes. There is a higher incidence of lymphoma
5. Scleroderma (Progressive systemic sclerosis)
Inflammation and progressive sclerosis of connective tissue of skin and viscera.
Antibodies
- Antinuclear antibodies.
- Rheumatoid factor. .
- Defect is cell mediated.
lesions
- Skin- depigmentation, sclerotic atrophy followed by cakinosis-claw fingers and mask face.
- Joints-synovitis with fibrosis
- Muscles- myositis.
- GIT- diffuse fibrous replacement of muscularis resulting in hypomotility and malabsorption
- Kidneys changes as in SLE and necrotising vasculitis.
- Lungs – fibrosing alveolitis.
- Vasculitis in any organ or tissue.
6.Wegener’s granulomatosis. A complex of:
- Necrotising lesions in upper respiratory tract.
- Disseminated necrotising vasculitis.
- Focal or diffuse glomerulitis.
Mechanism. Not known. It is classed with autoimmune diseases because of the vasculitis resembling other immune based disorders.
Keloids
General Pathology
Keloids
1. Characterized by a progressively enlarging scar.
2. Caused by an abnormal accumulation of collagen at the site of injury.
3. More common in African-Americans.
THE DECIDUOUS DENTITION
Dental Anatomy
THE DECIDUOUS DENTITION
I. The Deciduous Dentition
-It is also known as the primary, baby, milk or lacteal dentition.
diphyodont, that is, with two sets of teeth. The term deciduous means literally 'to fall off.'
There are twenty deciduous teeth that are classified into three classes. There are ten maxillary teeth and ten mandibular teeth. The dentition consists of incisors, canines and molars.
Immunohistochemistry
General Pathology
Immunohistochemistry
This is a method is used to detect a specific antigen in the tissue in order to identify the type of disease.
NEUROHISTOLOGY
Anatomy
NEUROHISTOLOGY
The nervous system develops embryologically from ectoderm, which forms the neural plate
Successive growth and folding of the plate results in the formation of the primitive neural tube.
The neuroblasts in the wall of the tube differentiates into 3 cell types:
Neurons: conduction of impulses
Neuroglial cells: connective tissue and support of CNS
Ependymal cells: Lines the lumen of the tube.
- Specialized neuro-ectodermal cells which lines the ventricles of the adult brain
- Essentially also a neuroglial cell
Basic Unit = neuron
Exhibits irritability (excitability) and conductivity
A typical neurons consists of:
Cell body : Has nucleus (karyon) and surrounding cytoplasm (perikaryon) which contains organelles cell's vitality
Dendrites: Several short processes
Axon:One large process
Terminates in twig like branches (telodendrons)
May also have collateral branches projecting along its course. These exit at nodes of Ranvier
Axon enveloped in a sheath, and together forms the nerve fiber
Classification:
May be done in different ways, i.e.
Functional = afferent, efferent, preganglionic, postganglionic, etc.
Morphological = shape, processes, etc
A typical morphological classification is as follows
a. Unipolar: Has one process only Not found in man
b. Bipolar (so-called ganglion cell):Has two processes Found in sensory systems, e.g. retina olfactory system
c. Multipolar: Has several process Most common in CNS
Cell bodies vary in shape, e.g. stellate (star) , pyramidal
d. Pseudo-unipolar: Essentially bipolar neurons, but processes have swung around cb and fused with each other. They therefore enter and leave at one pole of the cell.
Typical neuron:
- Has 2 or more dendrites
Close to the cb the cytoplasm of dendrites has Nissl granules as well as mitochondria
Only one axon Arises from axon hillock, Devoid of Nissl granules, Encased in myelin sheath
No additional covering except for occasional foot processes of neuroglial cells
May branch at right angles
Branches at a node of Ranvier is known as a collateral
Ends of axons break up into tree-like branches, known as telodendria
Axons may be short (Golgi Type II) e.g. internuncial long (Golgi Type I) e.g. pyramidal neuron
Nucleus Central position Large and spherical
Chromatin is extended and thus not seen in LM. This allows the nucleolus to be prominent
Cytoplasm (perikaryon)
Surrounds nucleus May be large or small, shape may be round, oval, flattened, pyramidal, etc
Contains aggregates Nissl granules(Bodies) which is also sometimes referred to as rhomboid flakes
aggregation of membranes and cisternae of rough endoplasmic reticulum (RER)
numerous ribosomes and polyribosomes scattered between cisternae
(Polyribosome = aggregate of free ribosomes clumped together)
responsible for ongoing synthesis of new cytoplasm and cytoplasmic substances
needed for conduction of impulses
highly active in cell protein synthesis
resultant loss of power to divide which is characteristic of neurons
- Golgi network surrounding nucleus (seen in EM only)
- Fibrils made up of:
- neurofilaments
- microtubules
Tubules involved in:
1. plasmic transport
2. maintenance of cell shape
3. essential for growth and elongation of axons and dendrites
Neurofilament:
1. provide skeletal framework
2. maintenance of cell shape
3. possible role in axonal transport
(Axonal [axoplasmic; plasmic] transport may be antero- or retrograde. Anterograde transport via neurotubules is fast and moves neurotransmitters. Retrograde transport is slow and is the reason why viruses and bacteria can attack and destroy cell bodies. E.g. polio in the ventral columns and syphilis in the dorsal columns).
- Numerous mitochondria
- Neurons lack ability to store glycogen and are dependent for energy on circulating glucose
Impulses are conducted in one direction only
Dendrites conduct towards the cb
Axons conduct away from cb
Synapses:
- Neurons interconnect by way of synapses
- Normally the telodendria of an axon synapse with the dendrites of a succeeding axon
axo-dendritic synapse
This is usually excitatory
- Other types of synapses are:
axo-axonic
May be excitatory and/or inhibitory
axo-somatic
May be excitatory and/or inhibitory
dendrodendritic
Usually inhibitory
- Synapses are not tight junctions but maintain a narrow space the so-called synaptic cleft
- The end of an telodendron is usually enlarged (bouton) and contains many synaptic vesicles,
mitochondrion, etc. Its edge that takes part in the synapse is known as the postsynaptic membrane and no
vesicles are seen in this area
- Synapses may be chemical (as above) or electrical as in the ANS supplying smooth muscle cells subjacent to adjacent fibres
Gray and White Matter of Spinal Cord:
- Gray matter contains:
- cb's (somas) of neurons
- neuroglial cells
- White matter contains:
- vast number of axons
- no cb's
- colour of white matter due to myelin that ensheathes axons
Myelin:
- Non-viable fatty material contains phospholipids, cholesterol and some proteins
- Soluble and not seen in H&E-sections because it has become dissolved in the process, thus leaving empty spaces around the axons
- Osmium tetroxide (OsO4) fixes myelin and makes it visible by staining it black. Seen as concentric rings in cross section
- Myelin sheath (neurolemma) is formed by two types of cells
- Within the CNS by Oligodendrocytes
- On the peripheral neurons system by Schwann cells
- Sheath is formed by being wrapped around the axon in a circular fashion by both types of cells
Neuroglial Cells:
- Forms roughly 40% of CNS volume
- May function as: 1. support
2. nurture ("feeding")
3. maintain
Types of glial cells:
Oligodendrocytes:
- Small dark stained dense nucleus
- Analogue of Schwann cell in peripheral nervous system
- Has several processes which forms internodal segments of several fibres (one cell ensheathes more than one axon)
- Provides myelin sheaths in CNS
- Role in nurturing (feeding) of cells
Astrocytes:
Protoplasmic astrocytes:
- found in gray matter
- round cell body
- large oval nucleus with prominent nucleolus
- large thick processes
- processes are short but profusely branched
- perivascular and perineurial foot processes
- sometimes referred to as mossy fibres
Fibrous Astrocytes:
- found in white matter
- polymorphic cells body
- large oval nucleus
- long thin processes
Microglia:
- Neural macrophages
- smallest of the glial cells
- intense dark stained nucleus
- conspicuously fine processes which has numerous short branches
Cerebral Cortex:
Consists of six layers which are best observed in the cortex of the hippocampus
From superficial to deep:
- Molecular layer:
- Has few cells and many fibres of underlying cells
- Outer granular layer:
- Many small nerve cells
- Pyramidal layer:
- Pyramidally-shaped cells bodies
- Inner granular layer:
- Smaller cells and nerve fibres
- Internal (inner) pyramidal layer:
- Pyramidal cells bodies
- Very large in the motor cortex and known as Betz-cells
- Polymorphic layer:
- Cells with many shapes
Cerebellar Cortex:
Consists of three layers
Connections are mainly inhibitory
From superficial to deep
- Outer molecular layer:
- Few cells and many fibres
- Purkinje layer:
- Huge flask-shaped cells that are arranged next to one another
- Inner granular layer:
- Many small nerve cells
Motor endplate:
Seen in periphery on striated muscle fibres
- known as boutons
- has no continuous myelin covering from the Schwann cells
- passes through perimysium of muscle fiber to "synapse"
- multiple synaptic gutter (fold) in sarcoplasma of muscle fiber beneath bouton
- contains numerous synaptic vesicles and mitochondria
Ganglia:
- Sensory Ganglia:
(e.g. trigeminal nerve, ganglia and dorsal root ganglia)
- No synapse (trophic unit)
- pseudo-unipolar neurons
- centrally located nucleus
- spherical smooth border
- conspicuous axon hillock
- Surrounded by cuboidal satellite cells (Schwann cells)
- Covered by spindle shaped capsular cells of delicate collagen which forms the endoneurium
- Visceral and Motor Ganglia (Sympathetic and Parasympathetic):
- Synapse present
- Ratio of preganglionic: postganglionic fibres
1. Sympathetic 1:30
Therefore excitatory and catabolic
2. Parasympathetic 1:2
Therefore anabolic
Except in Meissner and Auerbach's plexuses where ratio is 1:1000 '2 because of parasympathetic component's involvement in digestion
- Preganglionic axons are myelinated (e.g. white communicating rami)
- Postganglionic axon are non-myelinated (e.g. gray communicating rami)
- small multipolar cell body
- excentrally located nucleus
- Inconspicuous axon hillock
- satellite cells few or absent
- few capsular cells
Use of local anesthetics during pregnancy
Pharmacology
Use of local anesthetics during pregnancy
Local anesthetics (injectable)
Drug FDA category
Articaine C
Bupivacaine C
Lidocaine B
Mepivacaine C
Prilocaine B
Vasoconstrictors
Epinephrine 1:200,000 or 1:100,000 C (higher doses)
Levonordefrin 1:20,000 Not ranked
Local anesthetics (topical)
Benzocaine C
Lidocaine B
Ossification
Anatomy
Ossification
Intramembranous-found in the flat bones of the face
Mesenchymal cells cluster and form strands
Strands are cemented in a uniform network. Which is known as osteoid
Calcium salts are deposited; osteoid is converted to bone
Trabeculae are formed and make cancellous bone with open spaces known as marrow cavities
Periosteum forms on the inner and outer surfaces of the ossification centers
Surface bone becomes compact bone
Endochondral-primary type of ossification In the human