MDS PREP
Which of the following is NOT a method used to produce a recombinant hepatitis B vaccine?
1) Cloning the HBsAg gene in E. coli
2) Cloning the HBsAg gene in yeasts Saccharomyces cerevisae
3) Extracting and purifying HBsAg particles from infected human plasma
4) Using baculovirus vectors to produce HBsAg in insect cells
General Microbiology Answer: 1
While recombinant hepatitis B vaccines are produced using various methods, cloning the HBsAg gene in E. coli is not one of them. Instead, the gene is typically cloned in yeast or insect cells to produce the surface antigen for the vaccine.
Which of the following is a killed viral vaccine?
1) Sabin vaccine for poliomyelitis
2) Salk vaccine for poliomyelitis
3) MMR vaccine for measles, mumps, rubella
4) Varicella-zoster
General Microbiology Answer: 2
The Salk vaccine for poliomyelitis is a killed viral vaccine, effectively preventing the disease by using inactivated poliovirus.
Which of the following is true about the fermentation characteristics of Bacillus anthracis?
1) Ferments salicin
2) Produces H2S abundantly
3) Ferments glucose, maltose, lactose, and sucrose
4) All of the above
Bacillus anthracis ferments glucose, maltose, lactose, and sucrose, but does not ferment salicin or produce H2S abundantly.
Which of the following describes the colony morphology of Bacillus anthracis on an agar plate?
1) Smooth and shiny
2) Irregularly round with a frosted glass appearance
3) Small and translucent
4) Large and mucoid
Bacillus anthracis colonies on an agar plate typically appear irregularly round with a frosted glass appearance.
There are some enzymes that cause protein to coagulate rather than to produce simpler compund These are known as
1. lab enzymes
2. coagulase
3. proteolytic enzymes
4. wenmycin
Microbiology
Answer: 1
There are some enzymes that cause protein to coagulate rather than to produce simpler compund These are known as lab enzymes
1. Coagulase:
Definition: Coagulase is an enzyme produced by certain
bacteria, most notably Staphylococcus aureus. It catalyzes the
conversion of fibrinogen (a soluble plasma protein) into fibrin (an
insoluble protein), leading to the clotting of blood.
Function: The production of coagulase is a virulence
factor for Staphylococcus aureus, as it helps the bacteria evade
the host's immune system by forming a protective clot around the bacterial
cells. This clot can shield the bacteria from phagocytosis and other immune
responses.
Clinical Relevance: Coagulase is used in laboratory
tests to differentiate between pathogenic Staphylococcus aureus (coagulase-positive)
and non-pathogenic staphylococci (coagulase-negative).
2. Proteolytic Enzymes:
Definition: Proteolytic enzymes, also known as
proteases or peptidases, are enzymes that catalyze the breakdown of proteins
into smaller peptides or amino acids by cleaving the peptide bonds between
amino acids.
Function: These enzymes play crucial roles in various
biological processes, including digestion, protein turnover, cell signaling,
and immune responses. They are found in many organisms, including humans,
where they are involved in the digestion of dietary proteins in the stomach
and small intestine.
Types: Proteolytic enzymes can be classified based on
their mechanism of action (e.g., serine proteases, cysteine proteases,
aspartic proteases, and metalloproteases) and their substrate specificity.
Wenmycin is an antibiotic that is derived from the
bacterium Streptomyces wenyangensis. It belongs to the class of
compounds known as macrolide antibiotics, which are
characterized by their large lactone ring structure. Wenmycin has been studied
for its antimicrobial properties, particularly against certain types of
bacteria.
What is the principle behind the oxidase test used to identify bacteria?
1) The presence of specific antigens
2) The activity of certain bacterial oxidases
3) The growth of bacteria on selective media
4) The presence of enzymes that hydrolyze certain sugars
General Microbiology Answer: 2
The oxidase test is based on the presence of cytochrome c oxidase in bacteria. This enzyme catalyzes the oxidation of certain substrates, leading to a color change in the test medium, which indicates a positive result for oxidase activity.
Which of the following statements is true regarding Bacillus anthracis?
1) It is motile.
2) It is capsulated.
3) It grows in short chains.
4) It is susceptible to chloral hydrate.
Bacillus anthracis is capsulated, which is a key feature of its virulence.
Which of the following statements is true regarding the hemolysis of Bacillus anthracis?
1) Strong hemolysis is observed
2) Hemolysis is absent or weak
3) Complete hemolysis is typical
4) Variable hemolysis depending on the strain
Hemolysis of Bacillus anthracis is typically absent or weak, depending on the strain.