MDS PREP
The rate limiting step in glycolysis is catalyzed by:
1. Hexokinase
2. Phosphofructokinase
3. Enolase
4. Pyruvate kinase
Biochemistry
Answer: 2
The rate limiting step in glycolysis is catalyzed by Phosphofructokinase
The reaction used for estimating free amino, groups in proteins is:
1. Ninhydrin test
2. Deamination with HNO2
3. Biuret test
4. Formol titration
Biochemistry
Answer: 2
The reaction used for estimating free amino, groups in proteins is Deamination with HNO2
Urea formation take place in liver by:
1. Salvage pathway
2. Krebs cycle
3. Krebs Henseleit cycle
4. none
Biochemistry
Answer: 3
The synthesis of urea takes place in the liver by the process called ''Ornithine
cycle'' or "Urea cycle"
This cycle was discovered by Hans Krebs and Kurt Henseleit in the year 1932.
The Ornithine cycle is completed in 5 steps:
1. Conversion of ammonia into carbamoyl phosphate
2.The transfer of carbamoyl group from carbamoyl phosphate to ornithine
3. Releasing of the formed citrulline into the cytosol
4. Cleavage of argininosuccinate
5. Hydrolysis of Arginine: The final step involves the hydrolysis of Arginine
into urea and ornithine
Arginase is the sixth and final enzyme of this cycle.
Features of Vitamin C deficiency is due to defect of which enzyme
1. Lysyl hydroxylase
2. Lysyl oxidase
3. Procollagen peptidase
4. None of the above
Biochemistry
Answer: 1
Vitamin C deficiency is due to defect of Lysyl hydroxylase
Which of the following is a common dietary saturated fatty acid?
1. Oleic acid
2. Stearic acid
3. Linolenic acid
4. Arachidonic acid
Biochemistry
Answer: 4
Saturated fatty acids are a type of fat that has a chemical structure with a
single bond between each carbon atom in the fatty acid chain and no double
bonds. These types of fats are typically solid at room temperature. Out of the
given options, stearic acid (C18:0) is the only saturated fatty acid. The others
are:
1. Oleic acid (C18:1) is a monounsaturated fatty acid, which means it has one
double bond. It is commonly found in olive oil, canola oil, and avocados.
2. Linolenic acid (C18:3) is a polyunsaturated fatty acid, specifically an
omega-3 fatty acid, with three double bonds. It is found in plant sources like
flaxseeds, chia seeds, and walnuts.
3. Arachidonic acid (C20:4) is also a polyunsaturated fatty acid, an omega-6
fatty acid with four double bonds. It is found in animal fats, such as meat, and
in smaller amounts in some plant oils like sunflower oil and peanut oil.
Stearic acid, on the other hand, is a common saturated fatty acid found in
various animal fats and certain vegetable oils like coconut oil, palm kernel
oil, and cocoa butter. It is known for its role in the structure of cell
membranes and as a precursor for the biosynthesis of cholesterol in the body.
While it is important to consume fatty acids for good health, excessive
consumption of saturated fatty acids has been associated with higher levels of
LDL (low-density lipoprotein) cholesterol, which is often referred to as "bad"
cholesterol, and an increased risk of heart disease. However, it is important to
maintain a balanced diet that includes both saturated and unsaturated fatty
acids in moderation.
Aromatic amino acid is:
1. Tyrosine 2. Histidine
3. Arginine 4. Lysine
Biochemistry
Answer: 1
Tyrosine is Aromatic amino acid
Glutamate-pyruvate trans-aminase is predominantly present In:
1. Skeletal muscle
2. Cardiac muscle
3. Brain
4. Liver
Biochemistry
Answer: 4
Glutamate-pyruvate trans-aminase is predominantly present In Liver
Which of the following enzymes is active in adipocytes following a heavy meal?
1) Glycogen phosphorylase
2) Glycerol kinase
3) Hormone-sensitive triacylglyceride lipase
4) Phosphatidate phosphatase
Biochemistry Answer: 4
The enzyme phosphatidate phosphatase converts phosphatidic acid to diacylglycerol during synthesis of triacylglycerides.
The function of adipose tissue is the storage of fatty acids as triacylglycerols in times of plenty and the release of fatty acids during times of fasting or starvation.
Fatty acids taken in by adipocytes are stored by esterification to glycerol-3-phosphate. Glycerol-3-phosphate is derived almost entirely from the glycolytic intermediate dihydroxyacetone phosphate through the action of glycerol-3-phosphate dehydrogenase. Glycolytic enzymes are active in adipocytes during triglyceride synthesis, but those of glycogen degradation (low levels in adipocytes) and gluconeogenesis (ie, glucose-6-phosphatase) are not.
Glycerol kinase is not present to any great extent in adipocytes, so that glycerol freed during lipolysis is not used to reesterify the fatty acids being released.
The enzyme triacylglyceride lipase is turned on by phosphorylation by a cyclic AMP-dependent protein kinase following epinephrine stimulation.