Histology

Histology is the study of tissues.

A tissue is a group of cells with similar structure and function plus the extracellular substances located between the cells.

There are four basic types of tissues:

- Epitheliums

- Connective tissue

- Muscle tissue

- Nervous tissue

Cardiac Muscle

Fibres anastomose through cross bridges

Fibres are short, connected end to end at intercalated discs, also striated,  contract automatically

Light microscopic Structure:

Short fibres connected at intercalated disks,  85 - 100 µm long,  15 µm

same bands as in skeletal muscle,  1 or 2 nuclei - oval and central,  in perinuclear area is a sarcoplasmic reticulum, intercalated discs lie at the Z line

Electron microscopic structure:

 Between myofibrils lie the mitochondria,  2,5 µm long mitochondria,  dense cristae

and are as long as the sarcomere, fibres have more glycogen than skeletal muscle fibres

myofilaments, actin and myosin are the same as in skeletal muscle,  the sarcoplasmic reticulum differs in that there is no terminal sisterna. The sarcotubules end in little feet that

sit on the T-tubule

Intercalated Disc:

on Z lines,  fibres interdigitate,

 3 types of junctions in the disc

Transverse Part:

zonula adherens

desmosomes

Lateral Part:

Gap junctions (nexus) - for impulse transfer

Mechanism of Contraction:

slide - ratchet like in skeletal muscle, certain fibres are modified for conduction,  Impulses spread from cell to cell through gap junctions,  Purkinje cells are found in the AV bundle

they have less myofibrils,  lots of glycogen and intercalated discs

Connective tissue coverings:

Only endomycium in cardiac muscle,  Blood vessels, lymph vessels and nerves lie in the endomycium

CARTILAGE

There are 3 types:

Hyaline cartilage
Elastic cartilage
Fibrocartilage

Matrix is made up by: Hyaluronic acid

Proteoglycans

- In cartilage the protein core of the proteoglycan molecule binds through a linking protein to hyaluronic acid to form a proteoglycan aggregate which binds to the fibres

- In the matrix there are spaces, lacunae in which one to three of the cells of cartilage, chondrocytes, are found

- The matrix around the lacuna is the territorial matrix

- Type II collagen fibrils are embedded in the matrix

- The type of fiber depends on the type of cartilage

- Cartilage is surrounded by perichondrium which is a dense CT

- Apositional growth takes place in the perichondrium

- The fibroblasts of the perichondrium change to elliptic chondroblasts which later change to round chondrocytes

- Interstitial growth takes place around the lacunae

- Nutrients diffuse through the matrix to get to the chondrocytes   this limits the thickness of cartilage

Hyaline cartilage

Found: Rib cartilage,  articulating surfaces,  nose,  larynx, trachea, embryonic skeleton, Articulating cartilage has no perichondrium

 Bluish-white and translucent

Contains type II collagen that is not visible

Elastic cartilage

Found:  external auditory canal,  epiglottis

Similar to hyaline except that it contains many elastic fibres ,Yellow in colour,  Can be continuous with hyaline

Fibrocartilage

Found: Intervertebral disk, symphysis pubis

Always associated with dense CT,  Many collagen fibres in the matrix, No perichondrium

- Chondrocytes tend to lie in rows, Can withstand strong forces

Intramembranous ossification

• Flat bones develop in this way (bones of the skull)
• This type of bone development takes place in mesenchymal tissue
• Mesenchymal cells condense to form a primary ossification centre (blastema)
• Some of the condensed mesenchymal cells change to osteoprogenitor cells
• Osteoprogenitor cells change into osteoblasts which start to deposit bone
• As the osteoblasts deposit bone some of them become trapped in lacunae in the bone and then change into osteocytes
• Osteoblasts lie on the surface of the newly formed bone
• As more and more bone is deposited more and more osteocytes are formed from mesenchymal cells
• The bone that is formed is called a spicule
• This process takes place in many places simultaneously
• The spicules fuse to form trabeculae
• Blood vessels grow into the spaces between the trabeculae
• Mesenchymal cells in the spaces give rise to hemopoetic tissue
• This type of bone development forms the first phase in endochondral development
• It is also responsible for the growth of short bones and the thickening of long bones

The Pharynx

• The pharynx is the continuation of the digestive system from the oral cavity.
• It is a funnel-shaped fibromuscular tube that is the common route for both food and air.
• The pharynx is located posterior to the nasal and oral cavities, and the larynx.

• For the convenience of description, the pharynx is divided into three parts: (1) the nasopharynx, posterior to the nose and superior to the soft palate; (2) the oropharynx, posterior to the mouth; and (3) the laryngopharynx, posterior to the larynx.

• The pharynx is about 15 cm long.
• It extends from the base of the skull to the inferior border of the cricoid cartilage anteriorly, and to the inferior border of C6 vertebra posteriorly.
• It is widest (about 5 cm) opposite the hyoid bone and narrowest (about 1.5) at its inferior end, where it is continuous with the oesophagus.

• The posterior wall of the pharynx lies against the prevertebral fascia, with the potential retropharyngeal space between them.