Bell stage

The bell stage is known for the histodifferentiation and morphodifferentiation that takes place. The dental organ is bell-shaped during this stage, and the majority of its cells are called stellate reticulum because of their star-shaped appearance. Cells on the periphery of the enamel organ separate into three important layers. Cuboidal cells on the periphery of the dental organ are known as outer enamel epithelium.The cells of the enamel organ adjacent to the dental papilla are known as inner enamel epithelium. The cells between the inner enamel epithelium and the stellate reticulum form a layer known as the stratum intermedium. The rim of the dental organ where the outer and inner enamel epithelium join is called the cervical loop

Other events occur during the bell stage. The dental lamina disintegrates, leaving the developing teeth completely separated from the epithelium of the oral cavity; the two will not join again until the final eruption of the tooth into the mouth

The crown of the tooth, which is influenced by the shape of the internal enamel epithelium, also takes shape during this stage. Throughout the mouth, all teeth undergo this same process; it is still uncertain why teeth form various crown shapes—for instance, incisors versus canines. There are two dominant hypotheses. The "field model" proposes there are components for each type of tooth shape found in the ectomesenchyme during tooth development. The components for particular types of teeth, such as incisors, are localized in one area and dissipate rapidly in different parts of the mouth. Thus, for example, the "incisor field" has factors that develop teeth into incisor shape, and this field is concentrated in the central incisor area, but decreases rapidly in the canine area. The other dominant hypothesis, the "clone model", proposes that the epithelium programs a group of ectomesenchymal cells to generate teeth of particular shapes. This group of cells, called a clone, coaxes the dental lamina into tooth development, causing a tooth bud to form. Growth of the dental lamina continues in an area called the "progress zone". Once the progress zone travels a certain distance from the first tooth bud, a second tooth bud will start to develop. These two models are not necessarily mutually exclusive, nor does widely accepted dental science consider them to be so: it is postulated that both models influence tooth development at different times.Other structures that may appear in a developing tooth in this stage are enamel knots, enamel cords, and enamel niche.

Interarch relationship can be  viewed from a stationary (fixed) and a dynamic (movable ) perspective

1.Stationary Relationship

a) .Centric Relation is the most superior relationship of the condyle of the mandible to the articular fossa of the temporal bone as determined by the bones ligaments. and muscles of the temporomandibular joint; in an ideal dentition it is the same as centric occlusion

Centric occlusion is habitual occlusion where maximum intercuspation occurs

The characteristics of centric occlusion are

(1) Overjet: or that characteristic of maxillary teeth to overlap the mandibular teeth in a horizontal direction by 1 to 2 mm the maxilla arch is slightly larger; functions to protect the narrow edge of the incisors and provide for an intercusping relation of posterior teeth

(2) Overbite or that characteristic of maxillary anterior teeth to overlap the mandibular anterior teeth in a vertical direction by a third of the lower crown height facilitates scissor like function of incisors

(3) Intercuspation. or that characteristic of posterior teeth to intermesh in a faciolingual direction  The mandibular facial and maxillary lingual cusp  are centric cusps yhat contact interocclusally in the opposing arch

(4) Interdigitation, or that characteristic_of that tooth to  articulate with two opposing teeth (except for the mandibular central incisors and the maxillary last molars); a mandibular tooth occludes with the same tooth in the upper arch and the one mesial to it; a maxillary tooth occludes with the same tooth in the mandibular arch and the one distal to it.

2. Dynamic interarch relationshjps are result of functional mandibular movements that start and end with centric  occlusion during mastication

a. Mandibular movements are

(1) Depression (opening)

(2) Elevation (closing)

(3) Protrusion (thrust forward)

(4) Retrusion (bring back)

(5) Lateral movements right and left; one side is always the working side and one the balancing or nonworking side

b. Mandibular movements from centric occlusion are guided by the maxillary teeth

(1) Protrusion is guided by the incisors called incisal guidence

(2) Lateral movments are guided by the Canines on the working side in young, unworn dentitions (cuspid rise or cuspid protected occlusion); guided by incisors and posterior teeth in older worn. dentition (incisal/group guidance)

c. As mandibular movements commence from centric occlusion, posterior teeth should disengage in protrusion the posterior teeth on the balancing side should disengage in lateral movement

d. If tooth contact occurs where teeth should be disengaged, occlusal interference or premature contacts exist.

FUNCTIONS OF PERIODONTIUM

Tooth support
Shock absorber
Sensory (vibrations appreciated in the middle ear/reflex jaw opening)

Histology of the Pulp

PARTICIPATING CELLS

1. Odontoblasts (body and process)
Most distinctive cells of the pulp
Single layer
The cells are columnar in the coronal portion, cuboidal in the middle portion, flat in the apical portion

Individual odontoblasts communicate with each other via junctions. The number of odontoblasts corresponds to the number of dentinal tubules.
The lifespan of an odontoblast equals the one of a vital tooth.
The morphology of the odontoblasts reflects their functional activity.
(There are three stages that reflect the functional activity of a cell: active, transitional and resting)

The odontoblastic process

2. Fibroblasts
Most numerous cells
Produce collagen fibers and ground substance
Ground substance consists of: proteoglycans and glycoproteins
Again, active and resting cells
Fibroblasts have also capability to degrade collagen

3. Undifferentiated mesenchymal cells A pool of cells from which connective tissue cells can derive.
They are reduced with age.

4. Endothelial cells, Schwann cells, pericytes and immunocompetent cells

MATRIX

It is composed of fibers and ground substance
55% of the fibers are Type I collagen. 45% of the fibers are Type III collagen.
The ground substance is gelatinous in the coronal aspect and more fibrous in the apical.

VASCULARITY

Superior and inferior alveolar arteries that derive from the external carotids
Afferent side of the circulation: arterioles
Efferent side of the circulation: venules
Lymphatics

Small, blind, thin-walled vessels in the coronal region of the pulp and exit via one or two larger vessels.
 

Root Formation and Obliteration

1. In general, the root of a deciduous tooth is completely formed in just about one year after eruption of that tooth into the mouth.

2. The intact root of the deciduous tooth is short lived. The roots remain fully formed only for about three years.

3. The intact root then begins to resorb at the apex or to the side of the apex, depending on the position of the developing permanent tooth bud.

4. Anterior permanent teeth tend to form toward the lingual of the deciduous teeth, although the canines can be the exception. Premolar teeth form between the roots of the deciduous molar teeth

Amelogenesis and Enamel

Enamel is highly mineralized: 85% hydroxyapatite crystals
Enamel formation is a two-step process
The first step produces partially mineralized enamel: 30% (secretory)
The second step: Influx of minerals, removal of water and organic matrix (maturative)
Again, dentin is the prerequisite of enamel formation (reciprocal induction)
Stratum intermedium: high alkaline phosphatase activity
Differentiation of ameloblasts: Increase in glycogen contents

Formation of the enamel matrix
Enamel proteins, enzymes, metalloproteinases, phosphatases, etc.
Enamel proteins: amelogenins (90%), enamelin, tuftelin, and amelin
Amelogenins: bulk of organic matrix
Tuftelin: secreted at the early stages of amelogenesis (area of the DE junction)
Enamelin: binds to mineral
Amelin

Mineralization of enamel
 No matrix vesicles
Immediate formation of crystallites
Intermingling of enamel crystallites with dentin
"Soft" enamel is formed

Histologic changes

Differentiation of inner enamel epithelium cells. They become ameloblasts
Tomes' processes: saw-toothed appearance
Collapse of dental organ
Formation of the reduced enamel epithelium

Hard tissue formation (Amelogenesis )

Enamel formation is called amelogenesis and occurs in the crown stage of tooth development. "Reciprocal induction" governs the relationship between the formation of dentin and enamel; dentin formation must always occur before enamel formation. Generally, enamel formation occurs in two stages: the secretory and maturation stages. Proteins and an organic matrix form a partially mineralized enamel in the secretory stage; the maturation stage completes enamel mineralization.

In the secretory stage, ameloblasts release enamel proteins that contribute to the enamel matrix, which is then partially mineralized by the enzyme alkaline phosphatase. The appearance of this mineralized tissue, which occurs usually around the third or fourth month of pregnancy, marks the first appearance of enamel in the body. Ameloblasts deposit enamel at the location of what become cusps of teeth alongside dentin. Enamel formation then continues outward, away from the center of the tooth.

In the maturation stage, the ameloblasts transport some of the substances used in enamel formation out of the enamel. Thus, the function of ameloblasts changes from enamel production, as occurs in the secretory stage, to transportation of substances. Most of the materials transported by ameloblasts in this stage are proteins used to complete mineralization. The important proteins involved are amelogenins, ameloblastins, enamelins, and tuftelins. By the end of this stage, the enamel has completed its mineralization.