NEET MDS Lessons
Dental Anatomy
Dental Terminology.
Cusp: a point or peak on the occlusal surface of molar and premolar teeth and on the incisal edges of canines.
Contact: a point or area where one tooth is in contact (touching) another tooth
Cingulum: a bulge or elevation on the lingual surface of incisors or canines. It makes up the bulk of the cervical third of the lingual surface. Its convexity mesiodistally resembles a girdle encircling the lingual surface at the cervical.
Fissure: A linear fault that sometimes occurs in a developmental groove by incomplete or imperfect joining of the lobes. A pit is usually found at the end of a developmental groove or a place where two fissures intersect.
Lobe: one of the primary centers of formation in the development of the crown of the tooth.
Mamelon: A lobe seen on anterior teeth; any one of three rounded protuberances seen on the unworn surfaces of freshly erupted anterior teeth.
Ridge: Any linear elevation on the surface of a tooth. It is named according to its location or form. Examples are buccal ridges, incisal ridges, marginal ridges, and so on.
Marginal ridges are those rounded borders of enamel which form the margins of the surfaces of premolars and molars, mesially and distally, and the mesial and distal margins of the incisors and canines lingually.
Triangular ridges are those ridges which descend from the tips of the cusps of molars and premolars toward the central part of the occlusal surface. Transverse ridges are created when a buccal and lingual triangular ridge join.
Oblique ridges are seen on maxillary molars and are a companion to the distal oblique groove.
Cervical ridges are the height of contour at the gingival, on certain deciduous and permanent teeth.
Fossa: An irregular, rounded depression or concavity found on the surface of a tooth. A lingual fossa is found on the lingual surface of incisors. A central fossa is found on the occlusal surface of a molar. They are formed by the converging of ridges terminating at a central point in the bottom of a depression where there is a junction of grooves
Pit: A small pinpoint depression located at the junction of developmental grooves or at the terminals of these groops. A central pit is found in the central fossa on the occlusal surfaces of molars where developmental grooves join. A pit is often the site of the onset of Dental caries
Developmental groove: A sharply defined, narrow and linear depression formed during tooth development and usually separating lobes or major portions of a tooth.
A supplemental groove is also a shallow linear depression but it is usually less distinct and is more variable than a developmental groove and does not mark the junction of primary parts of a tooth.
Buccal and lingual grooves are developmental grooves found on the buccal and lingual surfaces of posterior teeth.
Tubercle: A small elevation produced by an extra formation of enamel. These occur on the marginal ridges of posterior teeth or on the cingulum of anterior teeth. These are deviations from the typical form.
Interproximal space: The triangular space between the adjacent teeth cervical to the contact point. The base of the triangle is the alveolar bone; the sides are the proximal surfaces of the adjacent teeth.
Sulcus:-An elongated valley or depression in the surface of a tooth formed by the inclines of adjacent cusp or ridges.
Embrasures: When two teeth in the same arch are in contact, their curvatures adjacent to the contact areas form spillway spaces called embrasures. There are three embrasures:
(1) Facial (buccal or labial)
(2) Occlusal or incisal
(3) Lingual
(NOTE: there are three embrasures; the fourth potential space is the interproximal space ).
Nerve and vascular formation
Frequently, nerves and blood vessels run parallel to each other in the body, and the formation of both usually takes place simultaneously and in a similar fashion. However, this is not the case for nerves and blood vessels around the tooth, because of different rates of development.
Nerve formation
Nerve fibers start to near the tooth during the cap stage of tooth development and grow toward the dental follicle. Once there, the nerves develop around the tooth bud and enter the dental papilla when dentin formation has begun. Nerves never proliferate into the enamel organ
Vascular formation
Blood vessels grow in the dental follicle and enter the dental papilla in the cap stage. Groups of blood vessels form at the entrance of the dental papilla. The number of blood vessels reaches a maximum at the beginning of the crown stage, and the dental papilla eventually forms in the pulp of a tooth. Throughout life, the amount of pulpal tissue in a tooth decreases, which means that the blood supply to the tooth decreases with age. The enamel organ is devoid of blood vessels because of its epithelial origin, and the mineralized tissues of enamel and dentin do not need nutrients from the blood.
CEMENTUM vs. BONE
Cementum simulates bone
1) Organic fibrous framework, ground substance, crystal type, development
2) Lacunae
3) Canaliculi
4) Cellular components
5) Incremental lines (also known as "resting" lines; they are produced by continuous but phasic, deposition of cementum)
Differences between cementum and bone
1) Cementum is not vascularized
2) Cementum has minor ability to remodel
3) Cementum is more resistant to resorption compared to bone
4) Cementum lacks neural component
5) Cementum contains a unique proteoglycan interfibrillar substance
6) 70% of bone is made by inorganic salts (cementum only 46%)
Relation of Cementum to Enamel at the Cementoenamel Junction (CEJ)
"OMG rule"
In 60% of the teeth cementum Overlaps enamel
In 30% of the teeth cementum just Meets enamel
In 10% of the teeth there is a small Gap between cementum and enamel
Clinical importance of cementum
1) Deposition of cementum continues throughout life.
The effects of the continuous deposition of cementum are the maintenance of total length of the tooth (good) and constriction of the apical foramen (bad).
2) With age, the smooth surface of cementum becomes more irregular due to calcification of some ligament fiber bundles. This is referred to as spikes.
Behavior of cementum in pathologic conditions
Formation and Eruption of Deciduous Teeth.
-Calcification begins during the fourth month of fetal life. By the end of the sixth month, all of the deciduous teeth have begun calcification.
-By the time the deciduous teeth have fully erupted (two to two and one half years of age), cacification of the crowns of permanent teeth is under way. First permanent molars have begun cacification at the time of birth. -Here are some things to know about eruption patterns:
(1) Teeth tend to erupt in pairs.
(2) Usually, lower deciduous teeth erupt first. Congenitally missing deciduous teeth is infrequent. Usually, the lower deciduous central incisors are thefirst to erupt thus initiating the deciduous dentition. The appearance of the deciduous second molars completes the deciduous dentition by 2 to 2 1/2 years of age.
- Deciduous teeth shed earlier and permanent teeth erupt earlier in girls.
- The orderly pattern of eruption and their orderly replacement by permanent teeth is important.
- order for eruption of the deciduous teeth is as follows:
(1) Central incisor.........Lower 6 ½ months, Upper 7 ½ months
(2) Lateral incisor.........Lower 7 months, Upper 8 months
(3) First deciduous molar...Lower 12-16 months, Upper 12-16 months
(4) Deciduous canine........Lower 16-20 months, Upper 16-20 months
(5) Second deciduous molar..Lower 20-30 months, Upper 20-30 months
Structure
There are 3 pairs
The functional unit is the adenomere.
The adenomere consists of secreting units and an intercalated duct, which opens, in a striated duct.
An secreting unit can be:
- mucous secreting
- serous secreting
THE SECRETING UNIT
THE CELLS
Serous cells
(seromucus cells=secrete also polysaccharides), They have all the features of a cell specialized for the synthesis, storage, and secretion of protein
Pyramidal, Nuclei are rounded and more centrally placed, In the basal 1/3 there is an accumulation of Granular EPR, In the apex there are proteinaceous secretory granules, Cells stain well with H & E (red), Between cells are intercellular secretory capillaries
Rough endoplasmic reticulum (ribosomal sites-->cisternae)
Prominent Golgi-->carbohydrate moieties are added
Secretory granules-->exocytosis
The secretory process is continuous but cyclic
There are complex foldings of cytoplasmic membrane
The junctional complex consists of: 1) tight junctions (zonula occludens)-->fusion of outer cell layer, 2) intermediate junction (zonula adherens)-->intercellular communication, 3)desmosomes-->firm adhesion
Mucus cells
Pyramidal, Nuclei are flattened and near the base, Have big clear secretory granules
Cells do not stain well with H & E (white)
Production, storage, and secretion of proteinaceous material; smaller enzymatic component
-more carbohydrates-->mucins=more prominent Golgi
-less prominent (conspicuous) rough endoplasmic reticulum, mitochondria
-less interdigitations
Myoepithelial cells
Star-shaped, Centrally located nucleus, Long cytoplasmic arms - bound to the secretory cells by desmosomes, Have fibrils like smooth muscle, Squeeze the secretory cell
One, two or even three myoepithelial cells in each salivary and piece body, four to eight processes
Desmosomes between myoepithelial cells and secretory cells myofilaments frequently aggregated to form dark bodies along the course of the process. The myoepithelial cells of the intercalated ducts are more spindled-shaped and fewer processes
Ultrastructure very similar to that of smooth muscle cells (myofilaments, desmosomal attachments)
Functions of myoepithelial cells
-Support secretory cells
-Contract and widen the diameter of the intercalated ducts
-Contraction may aid in the rupture of acinar cells of epithelial origin
Ductal system
Three classes of ducts:
Intercalated ducts
They have small diameter; lined by small cuboidal cells; nucleus located in the center. They have a well-developed RER, Golgi apparatus, occasionally secretory granules, few microvilli. Myoepithelial cells are also present. Intercalated ducts are prominent in salivary glands having a watery secretion (parotid).
Striated ducts
They have columnar cells, a centrally located nucleus, eosinophilic cytoplasm. Prominenty striations that refer to indentations of the cytoplasmic membrane with many mitochondria present between the folds. Some RER and some Golgi. The cells have short microvilli.
The cells of the striated ducts modify the secretion (hypotonic solution=low sodium and chloride and high potassium). There is also presence of few basal cells.
Terminal excretory ducts
Near the striated ducts they have the same histology as the striated ducts. As the duct reaches the oral mucosa the lining becomes stratified. In the terminal ducts one can find goblet cells, basal cells, clear cells. The terminal ducts alter the electrolyte concentration and add mucoid substance.
Connective tissue
Presence of fibroblasts, inflammatory cells, mast cells, adipose cells
Extracellular matrix (glycoproteins and proteoglycans)
Collagen and oxytalan fibers
Nerve supply
The innervation of salivary glands is very complicated. There is no direct inhibitory innervation. There are parasympathetic and sympathetic impulses, the parasympathetic are more prevalent.
The parasympathetic impulses may occur in isolation, evoke most of the fluid to be excreted, cause exocytosis, induce contraction of myoepithelial cells (sympathetic too) and cause vasodialtion. There are two types of innervation: epilemmal and hypolemmal. There are beta-adrenergic receptors that induce protein secretion and L-adrenergic and cholinergic receptors that induce water and electrolyte secretion.
Hormones can influence the function of the salivary glands. They modify the salivary content but cannot initiate salivary flow.
Age changes
Fibrosis and fatty degenerative changes
Presence of oncocytes (eosinophilic cells containing many mitochondria)
Clinical considerations
Role of drugs, systemic disorders, bacterial or viral infections, therapeutic radiation, obstruction, formation of plaque and calculus.
- Rich capillary networks surround the adenomeres.
Amelogenesis and Enamel
Enamel is highly mineralized: 85% hydroxyapatite crystals
Enamel formation is a two-step process
The first step produces partially mineralized enamel: 30% (secretory)
The second step: Influx of minerals, removal of water and organic matrix (maturative)
Again, dentin is the prerequisite of enamel formation (reciprocal induction)
Stratum intermedium: high alkaline phosphatase activity
Differentiation of ameloblasts: Increase in glycogen contents
Formation of the enamel matrix
Enamel proteins, enzymes, metalloproteinases, phosphatases, etc.
Enamel proteins: amelogenins (90%), enamelin, tuftelin, and amelin
Amelogenins: bulk of organic matrix
Tuftelin: secreted at the early stages of amelogenesis (area of the DE junction)
Enamelin: binds to mineral
Amelin
Mineralization of enamel
No matrix vesicles
Immediate formation of crystallites
Intermingling of enamel crystallites with dentin
"Soft" enamel is formed
Histologic changes
Differentiation of inner enamel epithelium cells. They become ameloblasts
Tomes' processes: saw-toothed appearance
Collapse of dental organ
Formation of the reduced enamel epithelium
Hard tissue formation (Amelogenesis )
Enamel formation is called amelogenesis and occurs in the crown stage of tooth development. "Reciprocal induction" governs the relationship between the formation of dentin and enamel; dentin formation must always occur before enamel formation. Generally, enamel formation occurs in two stages: the secretory and maturation stages. Proteins and an organic matrix form a partially mineralized enamel in the secretory stage; the maturation stage completes enamel mineralization.
In the secretory stage, ameloblasts release enamel proteins that contribute to the enamel matrix, which is then partially mineralized by the enzyme alkaline phosphatase. The appearance of this mineralized tissue, which occurs usually around the third or fourth month of pregnancy, marks the first appearance of enamel in the body. Ameloblasts deposit enamel at the location of what become cusps of teeth alongside dentin. Enamel formation then continues outward, away from the center of the tooth.
In the maturation stage, the ameloblasts transport some of the substances used in enamel formation out of the enamel. Thus, the function of ameloblasts changes from enamel production, as occurs in the secretory stage, to transportation of substances. Most of the materials transported by ameloblasts in this stage are proteins used to complete mineralization. The important proteins involved are amelogenins, ameloblastins, enamelins, and tuftelins. By the end of this stage, the enamel has completed its mineralization.