NEET MDS Lessons
Dental Anatomy
Histology of the Periodontal Ligament (PDL)
Embryogenesis of the periodontal ligament
The PDL forms from the dental follicle shortly after root development begins
The periodontal ligament is characterized by connective tissue. The thinnest portion is at the middle third of the root. Its width decreases with age. It is a tissue with a high turnover rate.
FUNCTIONS OF PERIODONTIUM
Tooth support
Shock absorber
Sensory (vibrations appreciated in the middle ear/reflex jaw opening)
The following cells can be identified in the periodontal ligament:
a) Osteoblasts and osteoclasts b) Fibroblasts, c) Epithelial cells
Rests of Malassez
d) Macrophages
e) Undifferentiated cells
f) Cementoblasts and cementoclasts (only in pathologic conditions)
The following types of fibers are found in the PDL
-Collagen fibers: groups of fibers
-Oxytalan fibers: variant of elastic fibers, perpendicular to teeth, adjacent to capillaries
-Eluanin: variant of elastic fibers
Ground substance
PERIODONTAL LIGAMENT FIBERS
Principal fibers
These fibers connect the cementum to the alveolar crest. These are:
a. Alveolar crest group: below CE junction, downward, outward
b. Horizontal group: apical to ACG, right angle
c. Oblique group: numerous, coronally to bone, oblique direction
d. Apical group: around the apex, base of socket
e. Interradicular group: multirooted teeth
Gingival ligament fibers
This group is not strictly related to periodontium. These fibers are:
a. Dentogingival: numerous, cervical cementum to f/a gingiva
b. Alveologingival: bone to f/a gingiva
c. Circular: around neck of teeth, free gingiva
d. Dentoperiosteal: cementum to alv. process or vestibule (muscle)
e. Transseptal: cementum between adjacent teeth, over the alveolar crest
Blood supply of the PDL
The PDL gets its blood supply from perforating arteries (from the cribriform plate of the bundle bone). The small capillaries derive from the superior & inferior alveolar arteries. The blood supply is rich because the PDL has a very high turnover as a tissue. The posterior supply is more prominent than the anterior. The mandibular is more prominent than the maxillary.
Nerve supply
The nerve supply originates from the inferior or the superior alveolar nerves. The fibers enter from the apical region and lateral socket walls. The apical region contains more nerve endings (except Upper Incisors)
Dentogingival junction
This area contains the gingival sulcus. The normal depth of the sulcus is 0.5 to 3.0 mm (mean: 1.8 mm). Depth > 3.0 mm is considered pathologic. The sulcus contains the crevicular fluid
The dentogingival junction is surfaced by:
1) Gingival epithelium: stratified squamous keratinized epithelium 2) Sulcular epithelium: stratified squamous non-keratinized epithelium The lack of keratinization is probably due to inflammation and due to high turnover of this epithelium.
3) Junctional epithelium: flattened epithelial cells with widened intercellular spaces. In the epithelium one identifies neutrophils and monocytes.
Connective tissue
The connective tissue of the dentogingival junction contains inflammatory cells, especially polymorphonuclear neutrophils. These cells migrate to the sulcular and junctional epithelium.
The connective tissue that supports the sulcular epithelium is also structurally and functionally different than the connective tissue that supports the junctional epithelium.
Histology of the Col (=depression)
The col is found in the interdental gingiva. It is surfaced by epithelium that is identical to junctional epithelium. It is an important area because of the accumulation of bacteria, food debris and plaque that can cause periodontal disease.
Blood supply: periosteal vessels
Nerve supply: periodontal nerve fibers, infraorbital, palatine, lingual, mental, buccal
Tooth development is commonly divided into the following stages: the bud stage, the cap, the bell, and finally maturation. The staging of tooth development is an attempt to categorize changes that take place along a continuum; frequently it is difficult to decide what stage should be assigned to a particular developing tooth. This determination is further complicated by the varying appearance of different histological sections of the same developing tooth, which can appear to be different stages.
Bud stage
The bud stage is characterized by the appearance of a tooth bud without a clear arrangement of cells. The stage technically begins once epithelial cells proliferate into the ectomesenchyme of the jaw. The tooth bud itself is the group of cells at the end of the dental lamina.
Alveolar bone (process)
1. The bone in the jaws that contains the teeth alveoli (sockets).
2. Three types of bone :
a. Cribriform plate (alveolar bone proper)
(1) Directly lines and forms the tooth socket. It is compact bone that contains many holes, allowing for the passage of blood vessels. It has no periosteum.
(2) Serves as the attachment site for PDL (Sharpey’s) fibers.
(3) The tooth socket is constantly being remodeled in response to occlusal forces. The bone laid down on the cribriform plate, which also provides attachment for PDL fibers, is known as bundle bone.
(4) It is radiographically known as the lamina dura.
b. Cortical (compact) bone
(1) Lines the buccal and lingual surfaces of the mandible and maxilla.
(2) Is typical compact bone with a periosteum and contains Haversian systems.
(3) Is generally thinner in the maxilla and thicker in the mandible, especially around the buccal area of the mandibular premolar and molar.
c. Trabecular (cancellous, spongy) bone
(1) Is typical cancellous bone containing Haversian systems.
(2) Is absent in the maxillary anterior teeth region.
3. Alveolar crest (septa)
a. The height of the alveolar crest is usually 1.5 to 2 mm below the CEJ junction.
b. The width is determined by the shape of adjacent teeth.
(1) Narrow crests—found between teeth with relatively flat surfaces.
(2) Widened crests—found between teeth with convex surfaces or teeth spaced apart.
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Maxillary (upper) teeth |
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Primary teeth |
Central |
Lateral |
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First |
Second |
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Initial calcification |
14 wk |
16 wk |
17 wk |
15.5 wk |
19 wk |
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Crown completed |
1.5 mo |
2.5 mo |
9 mo |
6 mo |
11 mo |
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Root completed |
1.5 yr |
2 yr |
3.25 yr |
2.5 yr |
3 yr |
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Mandibular (lower) teeth |
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Initial calcification |
14 wk |
16 wk |
17 wk |
15.5 wk |
18 wk |
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Crown completed |
2.5 mo |
3 mo |
9 mo |
5.5 mo |
10 mo |
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Root completed |
1.5 yr |
1.5 yr |
3.25 yr |
2.5 yr |
3 yr |
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The very first histological evidence of tooth development appear during the second month of intrauterine life. Calcification of deciduous incisors begins at 3-4 months in utero.
MAXILLARY SECOND MOLAR
The second molars are often called 12-year molars because they erupt when a child is about 12 years
Facial: The crown is shorter occluso-cervically and narrower mesiodistally whe compared to the first molar. The distobuccal cusp is visibly smaller than the mesiobuccal cusp. The two buccal roots are more nearly parallel. The roots are more parallel; the apex of the mesial root is on line with the with the buccal developmental groove. Mesial and distal roots tend to be about the same length.
Lingual: The distolingual cusp is smaller than the mesiolingual cusp. The Carabelli trait is absent.
Proximal: The crown is shorter than the first molar and the palatal root has less diverence. The roots tend to remain within the crown profile.
Occlusal: The distolingual cusp is smaller on the second than on the first molar. When it is much reduced in size, the crown outline is described as 'heart-shaped.' The Carabelli trait is usually absent. The order of cusp size, largest to smallest, is the same as the first but is more exaggerated: mesiolingual, mesiobuccal, distobuccal, and distolingual.
Contact Points; Height of Curvature: Both mesial and distal contacts tend to be centered buccolingually below the marginal ridges. Since themolars become shorter, moving from first to this molar, the contacts tend to appear more toward the center of the proximal surfaces.
Roots: There are three roots, two buccal and one lingual. The roots are less divergent than the first with their apices usually falling within the crown profile. The buccal roots tend to incline to the distal.
Note: The distolingual cusp is the most variable feature of this tooth. When it is large, the occlusal is somewhat rhomboidal; when reduced in size the crown is described as triangual or 'heart-shaped.' At times, the root may be fused.
Nutrition and tooth development
As in other aspects of human growth and development, nutrition has an effect on the developing tooth. Essential nutrients for a healthy tooth include calcium, phosphorus, fluoride, and vitamins A, C, and D. Calcium and phosphorus are needed to properly form the hydroxyapatite crystals, and their levels in the blood are maintained by Vitamin D. Vitamin A is necessary for the formation of keratin, as Vitamin C is for collagen. Fluoride is incorporated into the hydroxyapatite crystal of a developing tooth and makes it more resistant to demineralization and subsequent decay.
Deficiencies of these nutrients can have a wide range of effects on tooth development. In situations where calcium, phosphorus, and vitamin D are deficient, the hard structures of a tooth may be less mineralized. A lack of vitamin A can cause a reduction in the amount of enamel formation. Fluoride deficency causes increased demineralization when the tooth is exposed to an acidic environment, and also delays remineralization. Furthermore, an excess of fluoride while a tooth is in development can lead to a condition known as fluorosis.