Bell stage

The bell stage is known for the histodifferentiation and morphodifferentiation that takes place. The dental organ is bell-shaped during this stage, and the majority of its cells are called stellate reticulum because of their star-shaped appearance. Cells on the periphery of the enamel organ separate into three important layers. Cuboidal cells on the periphery of the dental organ are known as outer enamel epithelium.The cells of the enamel organ adjacent to the dental papilla are known as inner enamel epithelium. The cells between the inner enamel epithelium and the stellate reticulum form a layer known as the stratum intermedium. The rim of the dental organ where the outer and inner enamel epithelium join is called the cervical loop

Other events occur during the bell stage. The dental lamina disintegrates, leaving the developing teeth completely separated from the epithelium of the oral cavity; the two will not join again until the final eruption of the tooth into the mouth

The crown of the tooth, which is influenced by the shape of the internal enamel epithelium, also takes shape during this stage. Throughout the mouth, all teeth undergo this same process; it is still uncertain why teeth form various crown shapes—for instance, incisors versus canines. There are two dominant hypotheses. The "field model" proposes there are components for each type of tooth shape found in the ectomesenchyme during tooth development. The components for particular types of teeth, such as incisors, are localized in one area and dissipate rapidly in different parts of the mouth. Thus, for example, the "incisor field" has factors that develop teeth into incisor shape, and this field is concentrated in the central incisor area, but decreases rapidly in the canine area. The other dominant hypothesis, the "clone model", proposes that the epithelium programs a group of ectomesenchymal cells to generate teeth of particular shapes. This group of cells, called a clone, coaxes the dental lamina into tooth development, causing a tooth bud to form. Growth of the dental lamina continues in an area called the "progress zone". Once the progress zone travels a certain distance from the first tooth bud, a second tooth bud will start to develop. These two models are not necessarily mutually exclusive, nor does widely accepted dental science consider them to be so: it is postulated that both models influence tooth development at different times.Other structures that may appear in a developing tooth in this stage are enamel knots, enamel cords, and enamel niche.

Compensating curvatures of the individual teeth.

- the gentle curvature of the long axes of certain posterior teeth to exhibit a gentle curvature.

-These are probably analogous to the trabecular patterns seen in the femur and therefore reflect lines of stress experienced during function.

Posteruptive tooth movement.

These movements occur after eruption of the teeth into function in the oral cavity. These movements, known collectively as occlusomesial forces.

A. Continuous tooth eruption eruption of teeth after coming into occlusion. This process compensates for occlusal tooth wear.. Cementum deposition and progressive remodelling of the alveolar bone are the growth processes that provide for continuous tooth movement

B. Physiological mesial drift :Tthe tendency of permanent posterior teeth to migrate mesially in the dental arch both before and after they come into occlusion. Clinically, it compensates for proximal tooth wear.

(1) It describes the tendency of posterior teeth to move anteriorly.

(2) It applies to permanent teeth, not deciduous teeth.

(3) The distal tooth have the stronger is the tendency for drift.

(4) It compensates for proximal wear.

(5) In younger persons, teeth drift bodily; in older persons, they tip and rotate.

(6) Forces that cause it include occlusal forces, PDL contraction, and soft tissue pressures. There may be other more subtle factors as well.

Height  of Epithelial Attachment

The height of normal gingival tissue . mesiallv and distallv on approximating teeth, is directly dependent upon the height of the epithelial attachment on these teeth. Normal attachment follows the curvature of the cementoenamel junction if the teeth are jn proper, alignment and contact.

Amelogenesis and Enamel

Enamel is highly mineralized: 85% hydroxyapatite crystals
Enamel formation is a two-step process
The first step produces partially mineralized enamel: 30% (secretory)
The second step: Influx of minerals, removal of water and organic matrix (maturative)
Again, dentin is the prerequisite of enamel formation (reciprocal induction)
Stratum intermedium: high alkaline phosphatase activity
Differentiation of ameloblasts: Increase in glycogen contents

Formation of the enamel matrix
Enamel proteins, enzymes, metalloproteinases, phosphatases, etc.
Enamel proteins: amelogenins (90%), enamelin, tuftelin, and amelin
Amelogenins: bulk of organic matrix
Tuftelin: secreted at the early stages of amelogenesis (area of the DE junction)
Enamelin: binds to mineral
Amelin

Mineralization of enamel
 No matrix vesicles
Immediate formation of crystallites
Intermingling of enamel crystallites with dentin
"Soft" enamel is formed

Histologic changes

Differentiation of inner enamel epithelium cells. They become ameloblasts
Tomes' processes: saw-toothed appearance
Collapse of dental organ
Formation of the reduced enamel epithelium

Hard tissue formation (Amelogenesis )

Enamel formation is called amelogenesis and occurs in the crown stage of tooth development. "Reciprocal induction" governs the relationship between the formation of dentin and enamel; dentin formation must always occur before enamel formation. Generally, enamel formation occurs in two stages: the secretory and maturation stages. Proteins and an organic matrix form a partially mineralized enamel in the secretory stage; the maturation stage completes enamel mineralization.

In the secretory stage, ameloblasts release enamel proteins that contribute to the enamel matrix, which is then partially mineralized by the enzyme alkaline phosphatase. The appearance of this mineralized tissue, which occurs usually around the third or fourth month of pregnancy, marks the first appearance of enamel in the body. Ameloblasts deposit enamel at the location of what become cusps of teeth alongside dentin. Enamel formation then continues outward, away from the center of the tooth.

In the maturation stage, the ameloblasts transport some of the substances used in enamel formation out of the enamel. Thus, the function of ameloblasts changes from enamel production, as occurs in the secretory stage, to transportation of substances. Most of the materials transported by ameloblasts in this stage are proteins used to complete mineralization. The important proteins involved are amelogenins, ameloblastins, enamelins, and tuftelins. By the end of this stage, the enamel has completed its mineralization.

Dental Terminology.

Cusp: a point or peak on the occlusal surface of molar and premolar teeth and on the incisal edges of canines.

Contact: a point or area where one tooth is in contact (touching) another tooth

Cingulum: a bulge or elevation on the lingual surface of incisors or canines. It makes up the bulk of the cervical third of the lingual surface. Its convexity mesiodistally resembles a girdle  encircling the lingual surface at the cervical.

Fissure: A linear fault that sometimes occurs in a developmental groove by incomplete or imperfect joining of the lobes. A pit is usually found at the end of a developmental groove or a place where two fissures intersect.

Lobe: one of the primary centers of formation in the development of the crown of the tooth.

Mamelon: A lobe seen on anterior teeth; any one of three rounded protuberances seen on the unworn surfaces of freshly erupted anterior teeth.

Ridge: Any linear elevation on the surface of a tooth. It is named according to its location or form. Examples are buccal ridges, incisal ridges, marginal ridges, and so on.

Marginal ridges are those rounded borders of enamel which form the margins of the surfaces of premolars and molars, mesially and distally, and the mesial and distal margins of the incisors and canines lingually.

Triangular ridges are those ridges which descend from the tips of the cusps of molars and premolars toward the central part of the occlusal surface. Transverse ridges are created when a buccal and lingual triangular ridge join.

Oblique ridges are seen on maxillary molars and are a companion to the distal oblique groove.

Cervical ridges are the height of contour at the gingival, on certain deciduous and permanent teeth.

Fossa: An irregular, rounded depression or concavity found on the surface of a tooth. A lingual fossa is found on the lingual surface of incisors. A central fossa is found on the occlusal surface of a molar. They are formed by the converging of ridges terminating at a central point in the bottom of a depression where there is a junction of grooves

Pit: A small pinpoint depression located at the junction of developmental grooves or at the terminals of these groops. A central pit is found in the central fossa on the occlusal surfaces of molars where developmental grooves join. A pit is often the site of the onset of Dental  caries

Developmental groove: A sharply defined, narrow and linear depression formed during tooth development and usually separating lobes or major portions of a tooth.

A supplemental groove is also a shallow linear depression but it is usually less distinct and is more variable than a developmental groove and does not mark the junction of primary parts of a tooth.

Buccal and lingual grooves are developmental grooves found on the buccal and lingual surfaces of posterior teeth.

Tubercle: A small elevation produced by an extra formation of enamel. These occur on the marginal ridges of posterior teeth or on the cingulum of anterior teeth. These are deviations from the typical form.

Interproximal space: The triangular space between the adjacent teeth cervical to the contact point. The base of the triangle is the alveolar bone; the sides are the proximal surfaces of the adjacent teeth.

Sulcus:-An elongated valley or depression in the surface of a tooth formed by the inclines of adjacent cusp or ridges.

Embrasures: When two teeth in the same arch are in contact, their curvatures adjacent to the contact areas form spillway spaces called embrasures. There are three embrasures:

(1) Facial (buccal or labial)

(2) Occlusal or incisal

(3) Lingual

(NOTE: there are three embrasures; the fourth potential space is the interproximal space ).

Dental Formula, Dental Notation, Universal Numbering System

A. Dental Formula. The dental formula expresses the type and number of teeth per side

The Universal Numbering System. The rules are as follows:

1. Permanent teeth are designated by number, beginning with the last tooth on the upper right side, going on to the last tooth on the left side, then lower left to lower right

2. Deciduous teeth are designated by letter, beginning with the last tooth on the upper right side and proceeding in clockwise fashion

HISTOLOGIC CHANGES OF THE PULP

Regressive changes

Pulp decreases in size by the deposition of dentin.
This can be caused by age, attrition, abrasion, operative procedures, etc.
Cellular organelles decrease in number.

Fibrous changes

They are more obvious in injury rather than aging. Occasionally, scarring may also be apparent.

Pulpal stones or denticles

They can be: a)free, b)attached and/or c)embedded. Also they are devided in two groups: true or false. The true stones (denticles) contain dentinal tubules. The false predominate over the the true and are characterized by concentric layers of calcified material.

Diffuse calcifications

Calcified deposits along the collagen fiber bundles or blood vessels may be observed. They are more often in the root canal portion than the coronal area.

Histology of the Cementum

Cementum is a hard connective tissue that derives from ectomesenchyme.

Embryologically, there are two types of cementum:
Primary cementum: It is acellular and develops slowly as the tooth erupts. It covers the coronal 2/3 of the root and consists of intrinsic and extrinsic fibers (PDL).
Secondary cementum: It is formed after the tooth is in occlusion and consists of extrinsic and intrinsic (they derive from cementoblasts) fibers. It covers mainly the root surface.

Functions of Cementum

It protects the dentin (occludes the dentinal tubules)
It provides attachment of the periodontal fibers
It reverses tooth resorption

Cementum is composed of 90% collagen I and III and ground substance.
50% of cementum is mineralized with hydroxyapatite. Thin at the CE junction, thicker apically.