NEET MDS Lessons
Dental Anatomy
Dentinogenesis
Dentin formation, known as dentinogenesis, is the first identifiable feature in the crown stage of tooth development. The formation of dentin must always occur before the formation of enamel. The different stages of dentin formation result in different types of dentin: mantle dentin, primary dentin, secondary dentin, and tertiary dentin.
Odontoblasts, the dentin-forming cells, differentiate from cells of the dental papilla. They begin secreting an organic matrix around the area directly adjacent to the inner enamel epithelium, closest to the area of the future cusp of a tooth. The organic matrix contains collagen fibers with large diameters (0.1-0.2 μm in diameter). The odontoblasts begin to move toward the center of the tooth, forming an extension called the odontoblast process. Thus, dentin formation proceeds toward the inside of the tooth. The odontoblast process causes the secretion of hydroxyapatite crystals and mineralization of the matrix. This area of mineralization is known as mantle dentin and is a layer usually about 150 μm thick.
Whereas mantle dentin forms from the preexisting ground substance of the dental papilla, primary dentin forms through a different process. Odontoblasts increase in size, eliminating the availability of any extracellular resources to contribute to an organic matrix for mineralization. Additionally, the larger odontoblasts cause collagen to be secreted in smaller amounts, which results in more tightly arranged, heterogenous nucleation that is used for mineralization. Other materials (such as lipids, phosphoproteins, and phospholipids) are also secreted.
Secondary dentin is formed after root formation is finished and occurs at a much slower rate. It is not formed at a uniform rate along the tooth, but instead forms faster along sections closer to the crown of a tooth. This development continues throughout life and accounts for the smaller areas of pulp found in older individuals. Tertiary dentin, also known as reparative dentin, forms in reaction to stimuli, such as attrition or dental caries.
The dentin in the root of a tooth forms only after the presence of Hertwig's epithelial root sheath (HERS), near the cervical loop of the enamel organ. Root dentin is considered different than dentin found in the crown of the tooth (known as coronal dentin) because of the different orientation of collagen fibers, the decrease of phosphoryn levels, and the less amount of mineralization.
LOCATION OF THE TEETH
Normally, a human receives two sets of teeth during a lifetime.
The first (deciduous or primary) set consists of 20 teeth ("baby" teeth).
The second (permanent) set usually consists of 32 teeth. In each quadrant, there are eight permanent teeth: two incisors, one cuspid, two bicuspids, and three molars
The tooth positioned immediately to the side of the midline is the central incisor, so called because it occupies a central location in the arch.
To the side of the central incisor is the lateral incisor. Next is the cuspid, then the two bicuspids (the first bicuspid, followed by the second bicuspid). The last teeth are three molars. After the second bicuspid comes the first molar, followed by the second molar, followed by the third molar or more commonly called the "wisdom tooth."
Another method of describing the location of teeth is to refer to them as anterior or posterior teeth .
Anterior teeth are those located in the front of the mouth, the incisors, and the cuspids. Normally, these are the teeth that are visible when a person smiles.
The posterior teeth are those located in the back of the mouth-the bicuspids and molars.
lntraarch relationship refers to the alignment of the teeth within an arch
1. In an ideal alignment teeth should contact at their proximal crests of curvature. A continuous arch form is observed in occlusal view
Curves of the occlusal plane (a line connecting the cusp tips of the canines, premolars, and molars) are observed from the proximal view
Curve of Spee: anterior to posterior curve; for mandibular teeth the curve is concave and for maxillary teeth it is convex
Curve of Wilson- medial to lateral curve for mandibular teeth the curve is also convex and for the maxillary it is convex
2. Contact does not always exist Some permanent dentitions have normal spacing
Primary dentitions often have developmental spacing in the anterior area: some primary den titions have a pattern of spacing called primate spaces between the primary maxillary lateral incisors and canine and between the mandibular canine and first mo1ar
Disturbances to the intraarch alignment are described as
a. Qpen contact where interproximal space exist because of missing teeth oral habits, dental disease, or overdeveloped frena
b. where contact or position is at an unexpected area because of developmental disturbances, crowding, dental caries or periodontal ligament for their misplaced position: facial, lingual. mesial, supra(supraerupted) infra (infraerupted) and torso (rotated) version
Bell stage
The bell stage is known for the histodifferentiation and morphodifferentiation that takes place. The dental organ is bell-shaped during this stage, and the majority of its cells are called stellate reticulum because of their star-shaped appearance. Cells on the periphery of the enamel organ separate into three important layers. Cuboidal cells on the periphery of the dental organ are known as outer enamel epithelium.The cells of the enamel organ adjacent to the dental papilla are known as inner enamel epithelium. The cells between the inner enamel epithelium and the stellate reticulum form a layer known as the stratum intermedium. The rim of the dental organ where the outer and inner enamel epithelium join is called the cervical loop
Other events occur during the bell stage. The dental lamina disintegrates, leaving the developing teeth completely separated from the epithelium of the oral cavity; the two will not join again until the final eruption of the tooth into the mouth
The crown of the tooth, which is influenced by the shape of the internal enamel epithelium, also takes shape during this stage. Throughout the mouth, all teeth undergo this same process; it is still uncertain why teeth form various crown shapes—for instance, incisors versus canines. There are two dominant hypotheses. The "field model" proposes there are components for each type of tooth shape found in the ectomesenchyme during tooth development. The components for particular types of teeth, such as incisors, are localized in one area and dissipate rapidly in different parts of the mouth. Thus, for example, the "incisor field" has factors that develop teeth into incisor shape, and this field is concentrated in the central incisor area, but decreases rapidly in the canine area. The other dominant hypothesis, the "clone model", proposes that the epithelium programs a group of ectomesenchymal cells to generate teeth of particular shapes. This group of cells, called a clone, coaxes the dental lamina into tooth development, causing a tooth bud to form. Growth of the dental lamina continues in an area called the "progress zone". Once the progress zone travels a certain distance from the first tooth bud, a second tooth bud will start to develop. These two models are not necessarily mutually exclusive, nor does widely accepted dental science consider them to be so: it is postulated that both models influence tooth development at different times.Other structures that may appear in a developing tooth in this stage are enamel knots, enamel cords, and enamel niche.
Classification of Cementum
- Embryologically
Primary and secondary
2. According to cellular component
Acellular: Thin, Amorphous, First layer to seal the dentin tubules
Cellular: Thick, Better structure, Apical surface
Layers of cellular and acellular cementum alternate (randomly)
3. Based on the origin of the collagenous matrix
Extrinsic
Intrinsic
Mixed
4. Combined classification
a. Primary acellular intinsic fiber cementum
b. Primary acellualar extrinsic fiber cementum
c. Secondary cellular intrinsic fiber cementum
d. Secondary cellular mixed fiber cementum
e. Acellular afibrillar cementum
5. Depending on the location and patterning
Intermediate and mixed stratified cementum
Participating Cells
Cementoblasts
Active
Cells are round, plump with basophilic cytoplasm (rough endoplasmic reticulum)
Inactive
Cells have little cytoplasm
Cementocytes
- Cementocyte lacuna
- cementocyte canaliculus
Cells have fewer organelles compared to cementoblasts. They are found in lacunae and have numerous processes toward the periodontal ligament. Eventually they die due to avascularity
Cementicles
a) free
b) attached
c) embedded
Age changes in the dentition
I. After the teeth have reached full occlusion, microscopic tooth movements occur to compensate for wear at the contact area (Mesial Drift) and occlusal surfaces (by Deposition of cementum at the root apex)
2. Attrition of incisal ridges and cusp tips may be so severe that dentin may become exposed and intrinsically stained
3. Secondary dentin may be formed in response to dental caries, trauma, and aging and result in decreased pulp size and tooth sensation
HISTOLOGY OF THE ODONTOBLAST
Formation of Dentin
Mantle dentin: First formed dentin
Type I collagen with ground substance
Formation of the odontoblast process
Matrix vesicles
Appearance of hydroxyapatite crystals
Predentin
Primary physiologic (circumpulpal) dentin
All organic matrix is formed from odontoblasts
Smaller collagen fibers
Presence of phosphophoryn
Mineralization
Globular calcification
Interglobular dentin: Areas of incomplete calcification
Incremental lines of von Ebner: Daily, 4mm of organic matrix is deposited. Also every 5 days the arrangement of collagen fibers changes. This creates the incremental lines of von Ebner.
Intratubular dentin
Dentin tubules
S-shaped in the coronal aspect, straight in root dentin
Von Korff fibers
They are an artifact