Sterilization in Dental Practice

Sterilization is a critical process in dental practice, ensuring that all forms of life, including the most resistant bacterial spores, are eliminated from instruments that come into contact with mucosa or penetrate oral tissues. This guide outlines the accepted methods of sterilization, their requirements, and the importance of biological monitoring to ensure effectiveness.

Sterilization: The process of killing all forms of life, including bacterial spores, to ensure that instruments are free from any viable microorganisms. This is essential for preventing infections and maintaining patient safety.

Accepted Methods of Sterilization

There are four primary methods of sterilization commonly used in dental practices:

A. Steam Pressure Sterilization (Autoclave)

• Description: Utilizes steam under pressure to achieve high temperatures that kill microorganisms.
• Requirements:
  • Temperature: Typically operates at 121-134°C (250-273°F).
  • Time: Sterilization cycles usually last from 15 to 30 minutes, depending on the load.
  • Packaging: Instruments must be properly packaged to allow steam penetration.

B. Chemical Vapor Pressure Sterilization (Chemiclave)

• Description: Involves the use of chemical vapors (such as formaldehyde) under pressure to sterilize instruments.
• Requirements:
  • Temperature: Operates at approximately 132°C (270°F).
  • Time: Sterilization cycles typically last about 20 minutes.
  • Packaging: Instruments should be packaged to allow vapor penetration.

C. Dry Heat Sterilization (Dryclave)

• Description: Uses hot air to sterilize instruments, effectively killing microorganisms through prolonged exposure to high temperatures.
• Requirements:
  • Temperature: Commonly operates at 160-180°C (320-356°F).
  • Time: Sterilization cycles can last from 1 to 2 hours, depending on the temperature.
  • Packaging: Instruments must be packaged to prevent contamination after sterilization.

D. Ethylene Oxide (EtO) Sterilization

• Description: Utilizes ethylene oxide gas to sterilize heat-sensitive instruments and materials.
• Requirements:
  • Temperature: Typically operates at low temperatures (around 37-63°C or 98.6-145°F).
  • Time: Sterilization cycles can take several hours, including aeration time.
  • Packaging: Instruments must be packaged in materials that allow gas penetration.

Considerations for Choosing Sterilization Equipment

When selecting sterilization equipment, dental practices must consider several factors:

• Patient Load: The number of patients treated daily will influence the size and capacity of the sterilizer.
• Turnaround Time: The time required for instrument reuse should align with the sterilization cycle time.
• Instrument Inventory: The variety and quantity of instruments will determine the type and size of sterilizer needed.
• Instrument Quality: The materials and construction of instruments may affect their compatibility with certain sterilization methods.

Biological Monitoring

A. Importance of Biological Monitoring

• Biological Monitoring Strips: These strips contain spores calibrated to be killed when sterilization conditions are met. They serve as a reliable weekly monitor of sterilization effectiveness.

B. Process

• Testing: After sterilization, the strips are sent to a licensed reference laboratory for testing.
• Documentation: Dentists receive independent documentation of monitoring frequency and sterilization effectiveness.
• Failure Response: In the event of a sterilization failure, laboratory personnel provide immediate expert consultation to help resolve the issue.

Glass ionomer cement is a tooth coloured material 
Material was based on reaction between silicate glass powder & polyacrylicacid.
They bond chemically to tooth structure & release fluoride for relatively long period

CLASSIFICATION 

Type I. For luting

Type II. For restoration 

Type II.1 Restorative esthetic 

Type II.2 Restorative reinforced

Type III. For liner & bases

Type IV. Fissure & sealent

Type V. As Orthodontic cement

Type VI. For core build up

Physical Properties

1. Low solubility
2. Coefficient of thermal expansion similar to dentin
3. Fluoride release and fluoride recharge
4. High compressive strengths
5. Bonds to tooth structure
6. Low flexural strength
7. Low shear strength
8. Dimensional change (slight expansion) (shrinks on setting, expands with water sorption)
9. Brittle
10.Lacks translucency
11.Rough surface texture

Indications for use of Type II glass ionomer cements 

1) non-stress bearing areas 

2) class III and V restorations in adults 

3) class I and II restorations in primary dentition 

4) temporary or “caries control” restorations 

5) crown margin repairs 

6) cement base under amalgam, resin, ceramics, direct and indirect gold 

7) core buildups when at least 3 walls of tooth are remaining (after crown preparation)

Contraindications 

1) high stress applications I. class IV and class II restorations II. cusp replacement III. core build-ups with less than 3 sound walls remaining

Composition

 

Factors affecting the rate or setting

1. Glass composition:Higher Alumina – Silica ratio, faster set and shorter working time.
2. Particle Size: finer the powder, faster the set.
3. Addition of Tartaric Acid:-Sharpens set without shortening the working time.
4. Relative proportions of the constituents: Greater the proportion of glass and lower the proportion of water, the faster the set.
5. Temperature

Setting Time

Type 1 - 4-5 min
type II - 7 min

PROPERTIES 

Adhesion :

- Glass ionomer cement bonds chemically to the tooth structure->reaction occur between carboxyl group of poly acid & calcium of hydroxyl apatite.
 
- Bonding with enamel is higher than that of dentin ,due to greater inorganic content. 

Esthetics :
-GIC is tooth coloured material & available in different shades.
Inferior to composites.
They lack translucency & rough surface texture.
Potential for discolouration & staining.

Biocompatibilty :

- Pulpal response to glass ionomer cement is favorable. 
- Pulpal response is mild due to 
- High buffering capacity of hydroxy apatite. 
- Large molecular weight of the polyacrylic acid ,which prevents entry into dentinal tubules. 

a) Pulp reaction – ZOE < Glass Ionomer < Zinc Phosphate 

b) Powder:liquid ratio influences acidity 

c) Solubility & Disintegration:-Initial solubility is high due to leaching of intermediate products.The complete setting reaction takes place in 24 hrs, cement should be protected from saliva during this period.

Anticariogenic properties :
- Fluoride is released from glass ionomer at the time of mixing & lies with in matrix.
Fluoride can be released out without affecting the physical properties of cement.

ADVANTAGE DISADVANTAGE

Biologic Width and Drilling Speeds

In restorative dentistry, understanding the concepts of biologic width and the appropriate drilling speeds is essential for ensuring successful outcomes and maintaining periodontal health.

1. Biologic Width

Definition

• Biologic Width: The biologic width is the area of soft tissue that exists between the crest of the alveolar bone and the gingival margin. It is crucial for maintaining periodontal health and stability.
• Dimensions: The biologic width is ideally approximately 3 mm wide and consists of:
  • 1 mm of Connective Tissue: This layer provides structural support and attachment to the tooth.
  • 1 mm of Epithelial Attachment: This layer forms a seal around the tooth, preventing the ingress of bacteria and other irritants.
  • 1 mm of Gingival Sulcus: This is the space between the tooth and the gingiva, which is typically filled with gingival crevicular fluid.

Importance

• Periodontal Health: The integrity of the biologic width is essential for the health of the periodontal attachment apparatus. If this zone is compromised, it can lead to periodontal inflammation and other complications.

Consequences of Violation

• Increased Risk of Inflammation: If a restorative procedure violates the biologic width (e.g., by placing a restoration too close to the bone), there is a higher likelihood of periodontal inflammation.
• Apical Migration of Attachment: Violation of the biologic width can cause the attachment apparatus to move apically, leading to loss of attachment and potential periodontal disease.

2. Recommended Drilling Speeds

Drilling Speeds

• Ultra Low Speed: The recommended speed for drilling channels is between 300-500 rpm.
• Low Speed: A speed of 1000 rpm is also considered low speed for certain procedures.

Heat Generation

• Minimal Heat Production: At these low speeds, very little heat is generated during the drilling process. This is crucial for:
  • Preventing Thermal Damage: Low heat generation reduces the risk of thermal damage to the tooth structure and surrounding tissues.
  • Avoiding Pulpal Irritation: Excessive heat can lead to pulpal irritation or necrosis, which can compromise the health of the tooth.

Cooling Requirements

• No Cooling Required: Because of the minimal heat generated at these speeds, additional cooling with water or air is typically not required. This simplifies the procedure and reduces the complexity of the setup.

_{Pin size}

 

_{In general, increase in diameter of pin offers more retention but large sized pins can result in more stresses in dentin. Pins are available in four color coded sizes:}

 

Name	Pin diameter	Color code
·         Minuta	0.38 mm	Pink
·         Minikin	0.48mm	Red
·         Minim	0.61 mm	Silver
·         Regular	0.78 mm	Gold

 

_{Selection of pin size depends upon the following factors:}

 

_{·            Amount of dentin present}

_{·            Amount of retention required}

 

_{For most posterior restorations, Minikin size of pins is used because they provide maximum retention without causing crazing in dentin.}

_{A. Retention vs. Stress}

• _{Retention: Generally, an increase in the diameter of the pin offers more retention for the restoration.}
• _{Stress: However, larger pins can result in increased stresses in the dentin, which may lead to complications such as crazing or cracking of the tooth structure.}

_{2. Factors Influencing Pin Size Selection}

_{The selection of pin size depends on several factors:}

_{A. Amount of Dentin Present}

• _{Assessment: The amount of remaining dentin is a critical factor in determining the appropriate pin size. More dentin allows for the use of larger pins, while less dentin may necessitate smaller pins to avoid excessive stress.}

_{B. Amount of Retention Required}

• _{Retention Needs: The specific retention requirements of the restoration will also influence pin size selection. In cases where maximum retention is needed, larger pins may be considered, provided that sufficient dentin is available to accommodate them without causing damage.}

_{3. Recommended Pin Size for Posterior Restorations}

_{For most posterior restorations, the Minikin size pin (0.48 mm, color-coded red) is commonly used. This size provides a balance between adequate retention and minimizing the risk of causing crazing in the dentin.}

Mercury Release in Dental Procedures Involving Amalgam

Mercury is a key component of dental amalgam, and its release during various dental procedures has been a topic of concern due to potential health risks. Understanding the amounts of mercury released during different stages of amalgam handling is essential for dental professionals to implement safety measures and minimize exposure.

1. Mercury Release Quantification

A. Trituration

• Amount Released: 1-2 µg
• Description: Trituration is the process of mixing mercury with alloy particles to form a homogenous amalgam. During this process, small amounts of mercury can be released into the air, which can contribute to overall exposure.

B. Placement of Amalgam Restoration

• Amount Released: 6-8 µg
• Description: When placing an amalgam restoration, additional mercury may be released due to the manipulation of the material. This includes the handling and packing of the amalgam into the cavity preparation.

C. Dry Polishing

• Amount Released: 44 µg
• Description: Dry polishing of amalgam restorations generates the highest amount of mercury release among the listed procedures. The friction and heat generated during dry polishing can vaporize mercury, leading to increased exposure.

D. Wet Polishing

• Amount Released: 2-4 µg
• Description: Wet polishing, which involves the use of water to cool the restoration during polishing, results in significantly lower mercury release compared to dry polishing. The water helps to capture and reduce the amount of mercury vapor released into the air.

Refractory materials are essential in the field of dentistry, particularly in the branch of conservative dentistry and prosthodontics, for the fabrication of various restorations and appliances. These materials are characterized by their ability to withstand high temperatures without undergoing significant deformation or chemical change. This is crucial for the longevity and stability of the dental work. The primary function of refractory materials is to provide a precise and durable mold or pattern for the casting of metal restorations, such as crowns, bridges, and inlays/onlays.

Refractory materials include:

- Plaster of Paris: The most commonly used refractory material in dentistry, plaster is composed of calcium sulfate hemihydrate. It is mixed with water to form a paste that is used to make study models and casts. It has a relatively low expansion coefficient and is easy to manipulate, making it suitable for various applications.

- Dental stone: A more precise alternative to plaster, dental stone is a type of gypsum product that offers higher strength and less dimensional change. It is commonly used for master models and die fabrication due to its excellent surface detail reproduction.

- Investment materials: Used in the casting process of fabricating indirect restorations, investment materials are refractory and encapsulate the wax pattern to create a mold. They can withstand the high temperatures required for metal casting without distortion.

- Zirconia: A newer refractory material gaining popularity, zirconia is a ceramic that is used for the fabrication of all-ceramic crowns and bridges. It is extremely durable and has a high resistance to wear and fracture.

- Refractory die materials: These are used in the production of metal-ceramic restorations. They are capable of withstanding the high temperatures involved in the ceramic firing process and provide a reliable foundation for the ceramic layers.

The selection of a refractory material is based on factors such as the intended use, the required accuracy, and the specific properties needed for the final restoration. The material must have a low thermal expansion coefficient to minimize the thermal stress during the casting process and maintain the integrity of the final product. Additionally, the material should be able to reproduce the fine details of the oral anatomy and have good physical and mechanical properties to ensure stability and longevity.

Refractory materials are typically used in the following procedures:

- Impression taking: Refractory materials are used to make models from the patient's impressions.
- Casting of metal restorations: A refractory mold is created from the model to cast the metal framework.
- Ceramic firing: Refractory die materials hold the ceramic in place while it is fired at high temperatures.
- Temporary restorations: Some refractory materials can be used to produce temporary restorations that are highly accurate and durable.

Refractory materials are critical for achieving the correct fit and function of dental restorations, as well as ensuring patient satisfaction with the aesthetics and comfort of the final product.