Major Antimicrobial Proteins of Human Whole Saliva

Human saliva contains a variety of antimicrobial proteins that play crucial roles in oral health by protecting against pathogens, aiding in digestion, and maintaining the balance of the oral microbiome. Below is a summary of the major antimicrobial proteins found in human whole saliva, their functions, and their targets.

1. Non-Immunoglobulin (Innate) Proteins

These proteins are part of the innate immune system and provide immediate defense against pathogens.

• Lysozyme

  • Major Target/Function:
    • Targets gram-positive bacteria and Candida.
    • Functions by hydrolyzing the peptidoglycan layer of bacterial cell walls, leading to cell lysis.

• Lactoferrin

  • Major Target/Function:
    • Targets bacteria, yeasts, and viruses.
    • Functions by binding iron, which inhibits bacterial growth (iron sequestration) and has direct antimicrobial activity.

• Salivary Peroxidase and Myeloperoxidase

  • Major Target/Function:
    • Targets bacteria.
    • Functions in the decomposition of hydrogen peroxide (H2O2) to produce antimicrobial compounds.

• Histatin

  • Major Target/Function:
    • Targets fungi (especially Candida) and bacteria.
    • Functions as an antifungal and antibacterial agent, promoting wound healing and inhibiting microbial growth.

• Cystatins

  • Major Target/Function:
    • Targets various proteases.
    • Functions as protease inhibitors, helping to protect tissues from proteolytic damage and modulating inflammation.

2. Agglutinins

Agglutinins are glycoproteins that promote the aggregation of microorganisms, enhancing their clearance from the oral cavity.

• Parotid Saliva

  • Major Target/Function:
    • Functions in the agglutination/aggregation of a number of microorganisms, facilitating their removal from the oral cavity.

• Glycoproteins

  • Major Target/Function:
    • Functions similarly to agglutinins, promoting the aggregation of bacteria and other microorganisms.

• Mucins

  • Major Target/Function:
    • Functions in the inhibition of adhesion of pathogens to oral surfaces, enhancing clearance and protecting epithelial cells.

• β2-Microglobulin

  • Major Target/Function:
    • Functions in the enhancement of phagocytosis, aiding immune cells in recognizing and eliminating pathogens.

3. Immunoglobulins

Immunoglobulins are part of the adaptive immune system and provide specific immune responses.

• Secretory IgA

  • Major Target/Function:
    • Targets bacteria, viruses, and fungi.
    • Functions in the inhibition of adhesion of pathogens to mucosal surfaces, preventing infection.

• IgG

  • Major Target/Function:
    • Functions similarly to IgA, providing additional protection against a wide range of pathogens.

• IgM

  • Major Target/Function:
    • Functions in the agglutination of pathogens and enhancement of phagocytosis.

Indications for Stainless Steel Crowns in Pediatric Dentistry

• Extensive Tooth Decay:
  Stainless steel crowns (SSCs) are primarily indicated for teeth with significant decay that cannot be effectively treated with fillings. They provide full coverage, preventing further decay and preserving the tooth's structure.

• Developmental Defects:
  SSCs are beneficial for teeth affected by developmental conditions such as enamel dysplasia or dentinogenesis imperfecta, which make them more susceptible to decay.

• Post-Pulp Therapy:
  After procedures like pulpotomy or pulpectomy, SSCs are often used to protect the treated tooth, ensuring its functionality and longevity.

• High Caries Risk:
  For patients who are highly susceptible to caries, SSCs serve as preventive restorations, helping to protect at-risk tooth surfaces from future decay.

• Uncooperative Patients:
  In cases where children may be uncooperative during dental procedures, SSCs offer a quicker and less invasive solution compared to more complex treatments.

• Fractured Teeth:
  SSCs are also indicated for restoring fractured primary molars, which are crucial for a child's chewing ability and overall nutrition.

• Special Needs Patients:
  Children with special needs who may struggle with maintaining oral hygiene can benefit significantly from the durability and protection offered by SSCs.

Contraindications for Stainless Steel Crowns

1. Allergy to Nickel:

   • Some patients may have an allergy or sensitivity to nickel, which is a component of stainless steel. In such cases, alternative materials should be considered.

2. Severe Tooth Mobility:

   • If the tooth is severely mobile due to periodontal disease or other factors, placing a stainless steel crown may not be appropriate, as it may not provide adequate retention.

3. Inadequate Tooth Structure:

   • If there is insufficient tooth structure remaining to support the crown, it may not be feasible to place an SSC. This is particularly relevant in cases of extensive decay or fracture.

4. Active Dental Infection:

   • If there is an active infection or abscess associated with the tooth, it is generally advisable to treat the infection before placing a crown.

5. Patient Non-Compliance:

   • In cases where the patient is unlikely to cooperate with the treatment or follow-up care, the use of SSCs may not be ideal.

6. Aesthetic Concerns:

   • In anterior teeth, where aesthetics are a primary concern, parents or patients may prefer more esthetic options (e.g., composite crowns or porcelain crowns) over stainless steel crowns.

7. Severe Malocclusion:

   • In cases of significant malocclusion, the placement of SSCs may not be appropriate if they could interfere with the occlusion or lead to further dental issues.

8. Presence of Extensive Caries in Adjacent Teeth:

   • If adjacent teeth are also severely decayed, it may be more beneficial to address those issues first rather than placing a crown on a single tooth.

Classification of Oral Habits

Oral habits can be classified based on various criteria, including their nature, impact, and the underlying motivations for the behavior. Below is a detailed classification of oral habits:

1. Based on Nature of the Habit

• Obsessive Habits (Deep Rooted):

  • International or Meaningful:
    • Examples: Nail biting, digit sucking, lip biting.
  • Masochistic (Self-Inflicting):
    • Examples: Gingival stripping (damaging the gums).
  • Unintentional (Empty):
    • Examples: Abnormal pillowing, chin propping.

• Non-Obsessive Habits (Easily Learned and Dropped):

  • Functional Habits:
    • Examples: Mouth breathing, tongue thrusting, bruxism (teeth grinding).

2. Based on Impact

• Useful Habits:
  • Habits that may have a positive or neutral effect on oral health.
• Harmful Habits:
  • Habits that can lead to dental issues, such as malocclusion, gingival damage, or tooth wear.

3. Based on Author Classifications

• James (1923):

  • a) Useful Habits
  • b) Harmful Habits

• Kingsley (1958):

  • a) Functional Oral Habits
  • b) Muscular Habits
  • c) Combined Habits

• Morris and Bohanna (1969):

  • a) Pressure Habits
  • b) Non-Pressure Habits
  • c) Biting Habits

• Klein (1971):

  • a) Empty Habits
  • b) Meaningful Habits

• Finn (1987):

  • I. a) Compulsive Habits
  • b) Non-Compulsive Habits
  • II. a) Primary Habits

4. Based on Functionality

• Functional Habits:
  • Habits that serve a purpose, such as aiding in speech or feeding.
• Dysfunctional Habits:
  • Habits that disrupt normal oral function or lead to negative consequences.

Composition of Stainless Steel Crowns

Stainless steel crowns (SSCs) are primarily made from a specific type of stainless steel alloy, which provides the necessary strength, durability, and resistance to corrosion. Here’s a breakdown of the composition of the commonly used stainless steel crowns:

1. Stainless Steel (18-8) Austenitic Alloy:

• Common Brands: Rocky Mountain, Unitek
• Composition:
  • Iron: 67%
  • Chromium: 17%
  • Nickel: 12%
  • Carbon: 0.08 - 0.15%

This composition provides the crowns with excellent mechanical properties and resistance to corrosion, making them suitable for use in pediatric dentistry.

2. Nickel-Based Crowns:

• Examples: Inconel 600, 3M crowns
• Composition:
  • Iron: 10%
  • Chromium: 16%
  • Nickel: 72%
  • Others: 2%

Nickel-based crowns are also used in some cases, offering different properties and benefits, particularly in terms of strength and biocompatibility.

Rubber Dam in Dentistry

The rubber dam is a crucial tool in dentistry, primarily used for isolating teeth during various procedures. Developed by Barnum in 1864, it enhances the efficiency and safety of dental treatments.

Rationale for Using Rubber Dam

1. Maintains Clean and Visible Field

   • The rubber dam isolates the treatment area from saliva and blood, providing a clear view for the clinician.

2. Patient Protection

   • Prevents aspiration or swallowing of foreign bodies, such as dental instruments or materials, ensuring patient safety.

3. Clinician Protection

   • Reduces the risk of exposure to blood and saliva, minimizing the potential for cross-contamination.

4. Reduces Risk of Cross-Contamination

   • Particularly important in procedures involving the root canal system, where maintaining a sterile environment is critical.

5. Retracts and Protects Soft Tissues

   • The dam retracts the cheeks, lips, and tongue, protecting soft tissues from injury during dental procedures.

6. Increases Efficiency

   • Minimizes the need for patient cooperation and frequent rinsing, allowing for a more streamlined workflow.

7. Application of Medicaments

   • Facilitates the application of medicaments without the fear of dilution from saliva or blood.

8. Improved Properties of Restorative Material

   • Ensures that restorative materials set properly by keeping the area dry and free from contamination.

9. Psychological Benefit to the Patient

   • Provides a sense of security and comfort, as patients may feel more at ease knowing that the area is isolated and protected.

Rubber Dam Sheet Specifications

Rubber dam sheets are available in various thicknesses, which can affect their handling and application:

• Thin: 0.15 mm
• Medium: 0.20 mm
• Heavy: 0.25 mm
• Extra-Heavy: 0.30 mm
• Special Heavy: 0.35 mm

Sizes and Availability

• Rubber dam sheets can be purchased in rolls or prefabricated sizes, typically 5” x 5” or 6” x 6”.
• Non-latex rubber dams are available only in the 6” x 6” size.

Color Options

• Rubber dams come in various colors. Darker colors provide better visual contrast, while lighter colors can illuminate the operating field and facilitate the placement of radiographic films beneath the dam.

Surface Characteristics

• Rubber dam sheets have a shiny and a dull surface. The dull surface is typically placed facing occlusally, as it is less reflective and reduces glare, enhancing visibility for the clinician.

Wright's Classification of Child Behavior

1. Hysterical/Uncontrolled

   • Description: This behavior is often seen in preschool children during their first dental visit. These children may exhibit temper tantrums, crying, and an inability to control their emotions. Their reactions can be intense and overwhelming, making it challenging for dental professionals to proceed with treatment.

2. Defiant/Obstinate

   • Description: Children displaying defiant behavior may refuse to cooperate or follow instructions. They may argue or resist the dental team's efforts, making it difficult to conduct examinations or procedures.

3. Timid/Shy

   • Description: Timid or shy children may be hesitant to engage with the dental team. They might avoid eye contact, speak softly, or cling to their parents. This behavior can stem from anxiety or fear of the unfamiliar dental environment.

4. Stoic

   • Description: Stoic children may not outwardly express their feelings, even in uncomfortable situations. This behavior can be seen in spoiled or stubborn children, where their crying may be characterized by a "siren-like" quality. They may appear calm but are internally distressed.

5. Overprotective Child

   • Description: These children may exhibit clinginess or anxiety, often due to overprotective parenting. They may be overly reliant on their parents for comfort and reassurance, which can complicate the dental visit.

6. Physically Abused Child

   • Description: Children who have experienced physical abuse may display heightened anxiety, fear, or aggression in the dental setting. Their behavior may be unpredictable, and they may react strongly to perceived threats.

7. Whining Type

   • Description: Whining children may express discomfort or displeasure through persistent complaints or whining. This behavior can be a way to seek attention or express anxiety about the dental visit.

8. Complaining Type

   • Description: Similar to whining, complaining children vocalize their discomfort or dissatisfaction. They may frequently express concerns about the procedure or the dental environment.

9. Tense Cooperative

   • Description: These children are on the borderline between positive and negative behavior. They may show some willingness to cooperate but are visibly tense or anxious. Their cooperation may be conditional, and they may require additional reassurance and support.